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Effects of Rhizoma Gastrodiae on Cultured Mouse Spinal Motor Neurons Damaged by Hydrogen Peroxide  

Kim Hyung Su (School of Medicine, Wonkang University)
Lee Yang Suk (School of Medicine, Wonkang University)
Lee Whan Bong (School of Medicine, Wonkang University)
Son Il Hong (Institution of Wonkwang Science)
Lee Jae Kyoo (School of Medicine, Wonkang University)
Son Young Woo (School of Medicine, Wonkang University)
Lee Jung Hun (School of Medicine, Wonkang University)
Lee Kang Chang (Department of Graduate School of Oriental Medicine, Wonkang University)
Ryu Myeung Hwan (College of Oriental Medicine, Wonkang University)
Song Ho Joan (College of Oriental Medicine, Wonkang University)
Seong Kang Kyung (College of Oriental Medicine, Wonkang University)
Park Seung Taeck (Institution of Wonkwang Science)
Lee Kap Sang (College of Life Science and Natural Resources, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.1, 2002 , pp. 150-153 More about this Journal
Abstract
To elucidate the toxic effect of oxygen free radicals on cultured mouse spinal motor neurons damaged by hydrogen peroxide(H₂O₂)-induced neurotoxicity, we examined the neurotoxicity induced by oxygen radicals by NR assay when cultured spinal motor neurons were grown in the medium containing various concentrations of H₂O₂ for 6 hours. In addition, neuroprotective effects of herb extracts such Rhizoma Gastrodiae(RG), on H₂O₂-induced neurotoxicity in cultured spinal motor neurons were evaluated after cultured spinal motor neurons were preincubated with various concentrations of herb extract, RG for 2 hours before 50uM H₂O₂ for 6 hours. H₂O₂ decreased remarkably cell viability in dose-and time-dependent manner in these cultures, and also herb extract, RG increased cell viability of spinal motor neurons damaged by H₂O₂ in these cultures. From the above results, it is suggested that H₂O₂ was toxic in cultured spinal motor neurons derived from mouse, and RG was effective in blocking the neurotoxicity induced by oxidative stress in these cultures.
Keywords
Oxidative stress; Spinal motor neuron; Cell viability;
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