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http://dx.doi.org/10.17137/Korrae.2011.19.3.5

Effect of Benzalkonium Chloride on Biogas Potential of Pig Slurry  

Park, H. R. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Choi, H. L. (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Suresh, Arumuganainar (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
Publication Information
Journal of the Korea Organic Resources Recycling Association / v.19, no.3, 2011 , pp. 63-72 More about this Journal
Abstract
Benzalkonium chloride is most widely used in S. Korea as a disinfectant in livestock husbandry. Inhibition of biogas potentials were investigated with three different doses of benzalkonium chloride in swine slurry. The system was operated at batch mode. The inhibition rates were 10%, 30~40% and >70% at the dose of 10ppm, 40ppm and 80ppm, respectively assuming it was zero percent in case of no dose. Enzymatic activities were analyzed to determine the enzymatic type which was inhibited by benzalkonium chloride. The acid phosphatase, alkaline phosphatase and protease were shown negatively correlated with biogas potential. Correlation of ${\alpha}$-glucosidase and biogas potentials was observed not high (p<0.01, r=-0.426) while benzalkonium chloride (r=-0.853, p<0.01) and acid phosphatase (p<0.01) with biogas potentials were significantly and negatively correlated. The effect of benzalkonium chloride on Escherichia coli were also evaluated by disc diffusion method. As increase of benzalkonium concentration, inhibition zone of anaerobic bacteria was extended. It revealed that benzalkonium chloride significantly deteriorated biogas potential through inhibition of acetogenic bacteria.
Keywords
Benzalkonium chloride; Biogas; Methane; Enzymatic activity;
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