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http://dx.doi.org/10.12750/JARB.37.4.213

Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer  

Yeonmi Lee (Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University)
Eunju Kang (Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University)
Publication Information
Journal of Animal Reproduction and Biotechnology / v.37, no.4, 2022 , pp. 213-217 More about this Journal
Abstract
Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans. Some researchers showed partial somatic haploidization, but none observed embryo development. Our study attempted somatic haploidization using the modified somatic nuclear transfer (SCNT) protocol with various combinations of chemicals or proteins in mice. This study induced the proper segregation of somatic homologous chromosomes and full embryo development in vitro. Furthermore, somatic haploid embryos established embryonic stem cells and produced live births. The current review summarizes this recent study on the success of somatic haploidization and provides an overview of other related studies on somatic haploidization.
Keywords
chromosomal segregation; homologous chromosome; mice; SCNT; somatic haploidization;
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