Browse > Article
http://dx.doi.org/10.12750/JARB.37.3.169

High-dose lipopolysaccharide induced autophagic cell death in bovine mammary alveolar cells  

Park, Jin-Ki (Department of Livestock, Korea National University of Agricultures and Fisheries)
Yeo, Joon Mo (Department of Livestock, Korea National University of Agricultures and Fisheries)
Cho, Kwanghyun (Department of Livestock, Korea National University of Agricultures and Fisheries)
Park, Hyun-Jung (Department of Animal Biotechnology, College of Life Science, Sangji University)
Lee, Won-Young (Department of Livestock, Korea National University of Agricultures and Fisheries)
Publication Information
Journal of Animal Reproduction and Biotechnology / v.37, no.3, 2022 , pp. 169-175 More about this Journal
Abstract
Bovine mammary epithelial (MAC-T) cells are commonly used to study mammary gland development and mastitis. Lipopolysaccharide is a major bacterial cell membrane component that can induce inflammation. Autophagy is an important regulatory mechanism participating in the elimination of invading pathogens. In this study, we evaluated the mechanism underlying bacterial mastitis and mammary cell death following lipopolysaccharide treatment. After 24 h of 50 ㎍/mL lipopolysaccharide treatment, a significant decrease in the proliferation rate of MAC-T cells was observed. However, no changes were observed upon treatment of MAC-T cells with 10 ㎍/mL of lipopolysaccharide for up to 48 h. Thus, upon lipopolysaccharide treatment, MAC-T cells exhibit dose-dependent effects of growth inhibition at 10 ㎍/mL and death at 50 ㎍/mL. Treatment of MAC-T cells with 50 ㎍/mL lipopolysaccharide also induced the expression of autophagy-related genes ATG3, ATG5, ATG10, ATG12, MAP1LC3B, GABARAP-L2, and BECN1. The autophagy-related LC3A/B protein was also expressed in a dose-dependent manner upon lipopolysaccharide treatment. Based on these results, we suggest that a high dose of bacterial infection induces mammary epithelial cell death related to autophagy signals.
Keywords
autophagy; bovine mammary epithelial cell; cell death; lipopolysaccharide;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Li C, Li L, Chen K, Wang Y, Yang F, Wang G. 2019. UFL1 alleviates lipopolysaccharide-induced cell damage and inflammation via regulation of the TLR4/NF-κB pathway in bovine mammary epithelial cells. Oxid. Med. Cell. Longev. 2019:6505373.
2 Liu J, Zhang X, Yue S, Fu J, Chen J, Huang R, Shang P, Zhong K, Guo S, Jiao X, Zha G, Han L, Yang G, Li H, Wang Y. 2022. The anti-apoptotic and anti-autophagic effects of EPO through PI3K/Akt/mTOR signaling pathway in MAC-T cells. Res. Vet. Sci. 149:1-10.   DOI
3 Munz C. 2009. Enhancing immunity through autophagy. Annu. Rev. Immunol. 27:423-449.   DOI
4 Sun X, Luo S, Jiang C, Tang Y, Cao Z, Jia H, Xu Q, Zhao C, Loor JJ, Xu C. 2020. Sodium butyrate reduces bovine mammary epithelial cell inflammatory responses induced by exogenous lipopolysaccharide, by inactivating NF-κB signaling. J. Dairy Sci. 103:8388-8397.   DOI
5 Viguier C, Arora S, Gilmartin N, Welbeck K, O'Kennedy R. 2009. Mastitis detection: current trends and future perspectives. Trends Biotechnol. 27:486-493.   DOI
6 Ye X, Zhou XJ, Zhang H. 2018. Exploring the role of autophagy-related gene 5 (ATG5) yields important insights into autophagy in autoimmune/autoinflammatory diseases. Front. Immunol. 9:2334.   DOI
7 Cheng WN and Han SG. 2020. Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments - a review. Asian-Australas. J. Anim. Sci. 33:1699-1713.   DOI
8 Jing H, Chen Y, Liang W, Chen M, Qiu C, Guo MY. 2021. Effects of selenium on MAC-T cells in bovine mastitis: transcriptome analysis of exosomal mRNA interactions. Biol. Trace Elem. Res. 199:2904-2912.   DOI
9 Park HJ, Lee WY, Jeong HY, Song H. 2016. Regeneration of bovine mammary gland in immunodeficient mice by transplantation of bovine mammary epithelial cells mixed with Matrigel. Int. J. Stem Cells 9:186-191.   DOI
10 Li R, Fang H, Shen J, Jin Y, Zhao Y, Wang R, Fu Y, Tian Y, Yu H, Zhang J. 2021. Curcumin alleviates LPS-Induced oxidative stress, inflammation and apoptosis in bovine mammary epithelial cells via the NFE2L2 signaling pathway. Toxins (Basel) 13:208.   DOI
11 Wang H, Zhou Y, Zhu Q, Zang H, Cai J, Wang J, Cui L, Meng X, Zhu G, Li J. 2019. Staphylococcus aureus induces autophagy in bovine mammary epithelial cells and the formation of autophagosomes facilitates intracellular replication of Staph. aureus. J. Dairy Sci. 102:8264-8272.   DOI
12 Zhang L, Hou X, Sun L, He T, Wei R, Pang M, Wang R. 2018. Staphylococcus aureus bacteriophage suppresses LPS-induced inflammation in MAC-T bovine mammary epithelial cells. Front. Microbiol. 9:1614. (Erratum published 2018, Front. Microbiol. 9:2511)   DOI
13 Zhao X and Lacasse P. 2008. Mammary tissue damage during bovine mastitis: causes and control. J. Anim. Sci. 86(13 Suppl):57-65.   DOI
14 Geng N, Liu K, Lu J, Xu Y, Wang X, Wang R, Liu J, Liu Y, Han B. 2020. Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus. J. Microbiol. 58:320-329.   DOI
15 Huynh HT, Robitaille G, Turner JD. 1991. Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation. Exp. Cell Res. 197:191-199.   DOI
16 Lee WY, Park HJ, Yeo JM, Jeong HY, Song H. 2017. Enhancement of milk protein expression in mammary epithelial cells via co-culturing with preadipocyte cells. Biotechnol. Bioprocess Eng. 22:556-560.   DOI