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http://dx.doi.org/10.12750/JARB.35.2.207

Comparison of Cryotop and ReproCarreir products for cryopreservation of bovine blastocysts through survival rate and blastocysts quality  

Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University)
Yang, Seul-Gi (Department of Biotechnology, College of Engineering, Daegu University)
Park, Hyo-Jin (Department of Biotechnology, College of Engineering, Daegu University)
Kim, Ju Hwan (Modu Science)
Lee, Dong-Mok (Biomedical Manufacturing Technology Center, Korea Institute of Industrial Technology)
Woo, Seong-Min (Department of Biotechnology, College of Engineering, Daegu University)
Kim, Hyun-Jeong (Department of Biotechnology, College of Engineering, Daegu University)
Kim, Hyun Ah (Department of Biotechnology, College of Engineering, Daegu University)
Jeong, Jae-Hoon (Department of Biotechnology, College of Engineering, Daegu University)
Lee, Min Ji (Department of Biotechnology, College of Engineering, Daegu University)
Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
Publication Information
Journal of Animal Reproduction and Biotechnology / v.35, no.2, 2020 , pp. 207-213 More about this Journal
Abstract
Cryopreservation is used for blastocyst preservation of most mammalian embryos and is an important technique for breeding. We aimed to compare the efficiency of the cryopreservation method using the standard Cryotop device and the ReproCarrier device, a domestic product manufactured in Korea. The efficacy of the two devices was analyzed based on the survival rate, intracellular levels of reactive oxygen species (ROS), and apoptosis of the vitrified bovine blastocysts. The survival rates of the vitrified-warmed blastocysts were similar between the ReproCarrier group (58.4 ± 17.7%) and Cryotop group (59.9 ± 14.1%). Intracellular ROS levels and apoptotic index were determined by DCFDA staining and TUNEL assay. Changes in intracellular ROS levels, number of total nuclei, and cellular apoptosis of vitrified blastocysts after cryopreservation were not significantly different between the two groups. These results indicate that the ReproCarrier device method is as effective as the standard Cryotop method for vitrification of bovine blastocysts in vitro.
Keywords
bovine blastocyst; cryopreservation; Cryotop; ReproCarrier; vitrification;
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