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http://dx.doi.org/10.5187/jast.2020.62.6.854

Inhibitory effects of Porphyra dentata extract on 3T3-L1 adipocyte differentiation  

Choi, Su-Young (Department of Animal Science, Chonnam National University)
Lee, Su Yeon (Department of Food Science and Technology, Chonnam National University)
Jang, Da hye (Department of Food Science and Technology, Chonnam National University)
Lee, Suk Jun (Department of Biomedical Laboratory Science, College of Health & Medical Sciences, Cheongju University)
Cho, Jeong-Yong (Department of Food Science and Technology, Chonnam National University)
Kim, Sung-Hak (Department of Animal Science, Chonnam National University)
Publication Information
Journal of Animal Science and Technology / v.62, no.6, 2020 , pp. 854-863 More about this Journal
Abstract
This study was aimed to investigate the inhibitory effects of Porphyra dentata (P. dentata) extract on the adipogenesis of 3T3-L1 cells and evaluate its anti-obesity effect. The proliferation of 3T3-L1 cells and differentiation of adipocytes under treatment of P. dentata extract was examined by measuring the cell viability using alamarBlue assay and lipid droplets by Oil Red O staining. Results showed that P. dentata extract has no cytotoxicity effect and lipid droplets formation decreased in a concentration-dependent manner in 3T3-L1 cells. It has been confirmed that transcription factors affecting lipid accumulation and anti-adipogenic effects during cell differentiation are linked to P. dentata extract. We observed that P. dentata shows lowering the mRNA expression of peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα) that adipogenesis-associated key transcription factors and inhibiting adipogenesis in the early stages of differentiation. Treating the cells with P. dentata did not only suppressed PPARγ2 and C/EBPα but also significantly decreased the mRNA expression of adiponectin, Leptin, fatty acid synthase, adipocyte protein 2, and Acetyl-coA carboxylase 1. Overall, the P. dentata extract demonstrated inhibitory property in adipogenesis, which has a potential effect in anti-obesity in 3T3-L1 cells.
Keywords
3T3-L1; Adipocyte differentiation; Adipogenesis; Porphyra dentata; Anti-obesity;
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