Browse > Article
http://dx.doi.org/10.1186/s40781-016-0093-5

Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade Hanwoo  

Lee, Jun Young (Department of Animal Resources, Daegu University)
Jung, Yun Gil (Department of Animal Resources, Daegu University)
Seo, Byoung Boo (Department of Animal Resources, Daegu University)
Publication Information
Journal of Animal Science and Technology / v.58, no.3, 2016 , pp. 11.1-11.6 More about this Journal
Abstract
Background: This study was investigated the effects of culture media conditions on production of eggs fertilized in vitro of embryos from ovaries of high grade Korean native cow, Hanwoo. Methods: The IVMD 101 and IVF 100 were used for in vitro maturation of selected Hanwoo oocytes and In vitro embryo culture after in vitro fertilization, respectively. The IVMD 101 and IVD 101 were used for in vitro culture and completely free of serum. Results: The cleavage rates of 2-cell embryos in reference to Hanwoo oocytes were 86.7, 92.9, and 90.1 % in the control group, IVDM101 medium and IVD101 medium, respectively which indicates that the IVDM101 medium and IVD101 medium may result favorable outcomes. The in vitro development rates of blastocysts were 12.4, 38.4 and 32.4 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. For hatched blastocysts, it was 5.3, 33.9, and 28.6 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. Hence, more favorable results were expected for the hatched blastocysts in which the IVMD101 medium and IVD101 medium were used than the control group. Average cell numbers of blastocysts were 128.3, 165.7, and 163.6 in the groups of TCM-199 + 10 % FBS medium, IVMD 101 medium, and IVD 101 medium, respectively which clearly show that the IVMD 101 and IVD 101 medium consequence significantly higher cell numbers compared to the control group (i.e., TCM-199 +10 % FBS medium). Pregnancy rate after embryo transfer was 39.6 % when the serum free medium was used which is higher than that of the medium supplemented with serum (32.8 %). In addition, stillbirth rates were 4.9 % in the group of serum free medium whereas it was 13.6 % in the serum supplemented medium (13.6 %). Conclusions: Taken altogether, serum free media, the IVMD 101 and IVD 101 represented more favorable results in the embryo development rate of embryos, cell numbers of blastocyst, and pregnancy rate. Of note, the IVMD 101 medium showed better outcomes hence, it might be a better option for future applications for in vitro culture of bovine embryos.
Keywords
Hanwoo ovary; IVF; Serum free medium; Blastocyst; Embryo transfer;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Seidel GE. Critical review of embryo transfer procedures with cattle. Fert. and Embryo Develop. Plenum Press. 1980. 323-353.
2 Armstrong DT, Evans G. Factors influenceing success ot embryo transfer in sheep and goats. Theriogenology. 1983;19:31-42.   DOI
3 Schiewe MC, Bush M, Stuart LS, Wildt DR. Laparoscopic embryos transfer in domestic sheep: A preliminary study. Theriogenology. 1984;22:675-82.   DOI
4 Day BN. Embryo transfer swine. Theriogenology. 1979;43:190. (abstr).
5 Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA. Normal development following in vitro fertilzation in the cow. Biol Reprod. 1982;27:147-58.   DOI
6 Christensen LG. Use of embryo transfer in future cattle breeding schemes. Theriogenology. 1991;35:141-9.   DOI
7 Kajihara Y, Kometani N, Kobayashi S, Shitanaka Y, Koshiba Y, Shiraiwa K, Goto K. Pregnancy rates and births after co-culture of cumulus cells with bovine embryos derived from in vitro fertilization of in vitro matured follicular oocytes. Theriogenology. 1992;3:264.
8 Vieira AD, Forell F, Feltrin C, Rodrigues JL. Calves born after direct transfer of vitrified bovine in vitro-produced blastocysts derived from vitrified immature oocytes. Reprod Domest Anim. 2008;43(3):314-8.   DOI
9 Hirayama H, Moriyasu S, Kageyama S, Sawai K, Takahashi H, Geshi M, Fujii T, Koyama T, Koyama K, Miyamoto A, Matsui M, Minamihashi A. Enhancement of maternal recognition of pregnancy with parthenogeneticembryos in bovine embryo transfer. Theriogenology. 2014;81(8):1108-15.   DOI
10 Rosenkrans Jr CF, Zeng GQ, MCNamara GT, Schoff PK, First NL. Development of bovine embryos in vitro as affected by energy substrates. Biol Reprod. 1993;49:459-62.   DOI
11 Wright JM. Commercial Freezing of bovine embryos in straws. Theriogenology. 1985;23:17-29.   DOI
12 Wiemer KE, Watson AJ, Polanski V, Mckenna AI, Fick GH, Shultz GA. Effects of maturation and co-culturetreatments on the developmental capacity of early bovine embryos. Mol Reprod Dev. 1991;30:330-8.   DOI
13 Mermillod P, Wils C, Massip A, Dessy F. Collection of oocytes and production of blastocysts in vitro from individual, slaughtered cows. J Reprod Fert. 1992;96:717-23.   DOI
14 Hasler JF. Current status and potential of embryo transfer and reproductive technology in dairy cattle. J Dairy Sci. 1992;75:2857-79.   DOI
15 Blondin P, Sirard MA. Oocytes and follocular morphology as determining characteristics for developmental competence in bovine oocytes. Mol Reprod Dev. 1995;41:54-62.   DOI
16 Carolan C, Lonergan P, Khatir H, Mermillod P. In vitro production of bovine embryos using individual oocytes. Mol Reprd Dev. 1996;41:145-50.
17 Kim YJ, Chong GN, Lee HL, Cho SW, Kim YS, Yu IJ. Sex determination of biopsied Hanwoo embryos by polymerase chain reaction and embryo transfer with sexed blastocysts. Kor J Emb Trans. 2000;15(3):219-30.
18 Sel HS, Jung YK, Song HB. Developmental Competence of Oocytes Collected from the Ovaries of the Carcass of the High Meat Quality after IVM, IVF and IVC in Korean Native Cattle. Kor J Emb Trans. 2006;21(3):183-90.
19 Kim YJ, Kim HC, Seo SH, Jeong KN, Kim YS, Lee HR, Shin DS, Jo SW, Kim SH. Production of In-Vitro Fertilized Embryos and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle. Kor J Emb Trans. 2005;20(2):79-87.
20 Bondioli KM, Ellis SB, Pryor JH. The use of male specific chromosomal DNA fragments to determine the sex of bovine preimplantation embryos. Theriogenology. 1989;31:91-104.
21 Izadyare F, Colenbrander B, Bevers MM. Growth hormone stimulates in vitro bovine oocyte maturation and subsequent embryonic development. Theriogenology. 1996;33:669-75.
22 Kim YJ, Kim HC, Seo SH, Jeong KN, Kim YS, Lee HR, Shin DS, Jo SW, Kim SH. Production of In-Vitro Fertilized Embryos and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle. Kor J Emb Trans. 2005;20(2):79-87.
23 Cho SR, Choi SH, Choe CY, Son JK, Choi SH, Kim YJ, Lee PY, Yeon SH, Kim HJ, Son DS. Effect of In Vivo embryo production and embryo transfer following superovulation in Hanwoo. Kor J Emb Trans. 2010;25(2):141-4.
24 Hasler JF, Henderson WB, Hurtgen PJ, Jin ZQ, McCauley AD, Mower SA, Neely B, Skuey LS, Stockes JE, Trimmer SA. Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology. 1995;43:141-52.   DOI