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http://dx.doi.org/10.5187/JAST.2013.55.5.417

Comparison of Vitrification and Slow Freezing for the Cryopreservation of Chicken Primordial Germ Cell (Ogye)  

Kim, Sung Woo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Byun, Mijeong (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Do, Yoon Jung (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Han, Jae Yong (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University)
Kim, Dong Hun (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Seong, Hwan-Hoo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Kim, Hyun (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Publication Information
Journal of Animal Science and Technology / v.55, no.5, 2013 , pp. 417-425 More about this Journal
Abstract
We sought to provide a method for freezing and preserving primordial germ cells, or an avian germ cell of a bird, as a material for developmental engineering or species preservation. The aim of this study was to compare the efficacy of slow freezing with a vitrification method for the cryopreservation of chicken primordial germ cells (PGCs). PGCs obtained from the germinal gonad of day 5.5-6 day (stage 28) cultured chick embryos, using the MACS method, were classified into two groups: slow freezing and vitrification. We examined the viability of PGCs after Cryopreservation. Four freezing methods were compared with each other, including the following: Method 1: The PGCs were frozen by a programmed freezer in a plastic straw, including 2.0 M ethylene glycol (EG) as cryoprotective additive (slow freezing) Method 2: The PGCs were vitrified in a plastic straw, including 8.0 M EG, plus 7% polyvinylpyrrolidone (PVP) (rapid freezing). Method 3: The slow freezing was induced with a cryotube including 2.0 M EG Method 4: The PGCs were frozen in a cryotube including 10% dimethyl suloxide (DMSO) (rapid freezing). After freezing and thawing, survival rates of the frozen-thawed PGCs from Method 1 to 4were 76.4%, 70.6%, 80.5% and 78.1% (p<0.05), respectively. The slow freezing ($-80^{\circ}C$ programmed freezer) method may provide better survival rates of frozen-thawed PGCs than the vitrification method for the cryopreservation of PGCs. Therefore, these systems may contribute to the cryopreservation of a rare avian species.
Keywords
Primordial germ cells (PGCs); EG; Viability; Korean native chicken (Ogye); Slow freezing;
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