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http://dx.doi.org/10.5187/JAST.2012.54.2.133

Characteristics of α-Amylase and Protease Produced from Bacillus amyloliquefacies CNL-90 Isolated from Malt Grain  

Bae, Hyoung-Churl (Lab. of Milk Food Biochemistry and Biotechnology College of Agriculture and Life Sciences Chungnam National University)
Choi, Seong-Hyun (Research Institute, KG Si-Tech.)
Na, Seuk-Han (Chung Mi Bio Inc.)
Nam, Myoung-Soo (Lab. of Milk Food Biochemistry and Biotechnology College of Agriculture and Life Sciences Chungnam National University)
Publication Information
Journal of Animal Science and Technology / v.54, no.2, 2012 , pp. 133-139 More about this Journal
Abstract
A bacterium, identified as $Bacillus$ $amyloliquefacies$, CNL-90 using 16S rDNA analysis, was isolated from malt grain. The optimal activities of its ${\alpha}$-amylase and protease were observed at pH 6 and $60^{\circ}C$, and at pH 6 and $50^{\circ}C$, respectively although their activities remained stable at pH 7 and $40^{\circ}C$for ${\alpha}$-amylase and at pH 7 and $50^{\circ}C$ for protease. After solid-state fermentation of $B.$ $amyloliquefacies$, CNL-90 on wheat bran for 72hr or 144hr, the ${\alpha}$-amylase and protease activities were 170,000 and 290,000 units/kg, and 290,000 and 310,000 units/kg, respectively. The viable bacterial cell counts were $1.5{\times}10^9$ CFU/g and $2.2{\times}10^9$ CFU/g at 72hr and 144hr of the solid-state fermentation, respectively. A feeding trial with a total of 127 piglets was also conducted. The animals were divided into two groups: an experimental group fed with the fermented product (63 piglets) and a control group (64 piglets). The growth rate of the experimental group was 6.66% higher than that of the control group (P<0.05). The results of this study indicate that the ${\alpha}$-amylase and protease from $B.$ $amyloliquefacies$, CNL-90 can be used for industrial applications due to their activity in production of carbohydrate hydrolysates.
Keywords
$Bacillus$ $amyloliquefacies$ CNL-90; ${\alpha}$-amylase; Protease; Growth rate;
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