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http://dx.doi.org/10.5187/JAST.2012.54.1.59

Assessing Biological Safety of the Hanwoo Serum Obtained During Slaughtering Process  

Kim, Min-Soo (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University)
Yu, Ji-Eun (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University)
Min, Kyung-Ho (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University)
Kim, Ji-Hoe (School of Biotechnology, Yeungnam University)
Choi, In-Ho (School of Biotechnology, Yeungnam University)
Nahm, Sang-Soep (Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University)
Publication Information
Journal of Animal Science and Technology / v.54, no.1, 2012 , pp. 59-63 More about this Journal
Abstract
Bovine serum contains various nutrients and growth factors that can be potentially used in biological experiments, drug manufacturing process and food industry. However, almost all the bovine blood has been wasted during slaughter process in Korea, thus there is a high demand for alternative uses of the wasted sera. In order to produce high quality and safe sera, it is necessary to screen zoonotic pathogens as well as other microbial contaminants to prevent any downstream contamination. The present research has been undertaken to assess biological safety of Hanwoo sera by determining microbiological contamination during slaughtering and handling processes. Serological tests have been performed to detect bacteria, mycoplasma and virus contamination in total of 52 Hanwoo sera. No sera were found to be contaminated with mycoplasma or virus, but only two sera were found to be contaminated with Bacillus thuringiensis. The present result shows that Hanwoo sera obtained from slaughtering process are biologically safe and have potentials to be developed as a biological reagent. Moreover, the methods employed in our study may provide basic standard for microbiological screening methods once wasted Hanwoo sera gain industrial values.
Keywords
Hanwoo; Bovine serum; Zoonoses; Biological safety;
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1 USDA. 2011. Animal and Plant Health Inspection Service: Part 113.53 Requirements for ingredients of animal origin used for production of biologics, United States Department of Agriculture, Washington D.C, USA.
2 Lee, DM., Choi, MS., Woo, GI., Shin, YM., Lee, KH., Chun, YP., Chun, T. and Choi, I. 2009. Effect of gender-specific bovine serum supplemented medium on cell culture. J Anim Sci Technol 51:413-420.   DOI   ScienceOn
3 Lee, DM., Bajracharya, P., Lee, EJ., Kim, JE., Lee, HJ., Chun, T., Kim, J., Cho, KH., Chang, J., Hong, S. and Choi, I. 2011. Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation. In Vitro Cell Dev Biol Anim. 47:438-444.   DOI   ScienceOn
4 Macfarlane, DE. and Sommerville, RG. 1969. VERO cells (Cercopithecus aethiops kidney)--growth characteristics and viral susceptibility for use in diagnostic virology. (Brief report). Arch Gesamte Virusforsch 27:379-385.   DOI
5 McGarrity, GJ., Vanaman, V. and Sarama, J. 1984. Cytogenetic effects of mycoplasmal infection of cell cultures: a review. In Vitro 20:1-18.   DOI   ScienceOn
6 Muhlradt, PF., Meyer, H. and Jansen, R. 1996. Identification of S-(2,3-dihydroxypropyl) cystein in a macrophage-activating lipopeptide from Mycoplasma fermentans. Biochemistry 35:7781- 7786.   DOI   ScienceOn
7 Nanda, PK., Swain, P., Nayak, SK., Dash, S., Routray, P., Swain, SK. and Patra, BC. 2009. Goat serum as an alternative to establish cell culture from Indian major carp, Cirrhinus mrigala. In Vitro Cell Dev Biol Anim 45:148-151.   DOI   ScienceOn
8 Nijweide, PJ. and Burger, EH. 1990. Mechanism of bone formation in vitro. In The osteoblast (Ed. B. K. Hall), Telford Press, Caldwell, New Jersey. 1:303.
9 Roh, JY., Choi, JY., Li, MS., Jin, BR. and Je, YH. 2007. Bacillus thuringiensis as a specific, safe, and effective tool for insect pest control. J Microbiol Biotechnol 17:547-559.
10 Sinha-Hikim, I., Roth, SM., Lee, MI. and Bhasin, S. 2003. Testosterone-induced muscle hypertrophy is associated with an increase in satellite cell number in healthy, young men. Am J Physiol Endocrinol Metab 285:E197-205.   DOI
11 The Korean Society of Veterinary Public Health. 1996. Chapter 21. Meat hygiene, Veterinary Public Health, 3rd Ed. Munundang, pp361-448.
12 Armer, H., Moffat, K., Wileman, T., Belsham, G. J., Jackson, T., Duprex, W. P., Ryan, M. and Monaghan, P. 2008. Foot-and-mouth disease virus, but not bovine enterovirus, targets the host cell cytoskeleton via the nonstructural protein 3Cpro. J Virol 82:10556-10566.   DOI   ScienceOn
13 Govorkova, EA., Murti, G., Meignier, B., de Taisne, C. and Webster, RG. 1996. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol 70:5519-5524.
14 Hirata, F., Yoshida, M., Niwa, Y., Okouchi, M., Okayama, N., Takeuchi, Y., Itoh, M. and Ogura, Y. 2005. Insulin enhances leukocyte-endothelial cell adhesion in the retinal microcirculation through surface expression of intercellular adhesion molecule-1. Microvasc Res 69:135-141.   DOI   ScienceOn
15 Hirsh, D., MacLachlan, N. and Wlaker, R. 1999. Chapter 40. Mollicutes, Veterinary Microbiology, 2nd edition, Blackwell publishing, pp 240-249.
16 Kim, J., Kim, M., Nahm, SS., Lee, DM., Pokharel, S. and Choi, I. 2011. Characterization of gender-specific bovine serum. Animal Cells and Systems 15:147-154.   DOI
17 Houston, F., Foster, JD., Chong, A., Hunter, N. and Bostock, CJ. 2000. Transmission of BSE by blood transfusion in sheep. Lancet 356:999-1000.   DOI   ScienceOn
18 Ifediba, T. and Vanderberg, J. P. 1980. Peptones and calf serum as a replacement for human serum in the cultivation of Plasmodium falciparum. J Parasitol 66:236-239.   DOI   ScienceOn
19 Jang, Y., Kim. H., Lee M., Baek, H. and Choe, N. 2011. Utilization and hygiene status of animal blood from slaughterhouse in Korea. Kor J Vet Publ HLth 35:73-79.