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http://dx.doi.org/10.5187/JAST.2010.52.4.265

Gene Expression of Candidate Genes Involved in Fat Metabolism During In vitro Adipogenic Differentiation of Bovine Mesenchymal Stem Cell  

Kim, Sung-Kon (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Kim, Nam-Kuk (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Yoon, Du-Hak (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Kim, Tae-Hun (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Yang, Boo-Keun (Dept. of Animal Resources Science, College of Animal Life Science, Kangwon National University)
Lee, Hyun-Jeong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of Animal Science and Technology / v.52, no.4, 2010 , pp. 265-270 More about this Journal
Abstract
Adipogenesis has been one of the most intensely studied models of cellular differentiation. During adipogenesis, differential expression of many adipogenesis related genes lead to profound changes in cellular, morphological, and physiological characteristics of the differentiating cells. The aim of the present study was to examine the expression levels of adipogenic candidate genes, cAMP early repressor (ICER), nephroblastoma over-expressed protein (NOV), heat shock protein beta 1 (HSPB1) and succinate dehydrogenase (SDH), during adipogenesis of bovine mesenchymal stem cells (BMSC). The BMSC were cultured in DMEM / low glucose medium with adipogenic inducers for 6 days and the expression of various candidate genes which seemed related to adipogenesis were measured by real-time PCR. This study showed that the expression of peroxisome proliferator activated receptor ${\gamma}$(PPAR${\gamma}$) and fatty acid binding protein 4 (FABP4) genes as adipogenic indicators were increased to 3.11 and 3.11 folds on day 6 than on day 0, respectively (p<0.05). To determine whether candidate genes were related to adipogenesis, the expression levels of ICER, NOV, HSPB1, and SDH genes were measured during adipogenesis in BMSC. Our results showed that the expression level of ICER gene was significantly increased to 4.12 folds (0.01729 vs. 0.07138; p<0.05), whereas NOV, HSPB1, and SDH genes were decreased to 2.89, 3.18 and 2.36 folds, respectively, on day 6 when compared to day 0. These results suggest that these candidate genes have stimulatory or inhibitory effects on adipogenesis in BMSC, indicating that these genes may be directly or indirectly related to the adipogenic event of adipose precursor cells.
Keywords
Bovine mesenchymal stem cell; Adipogenesis; Differential expression;
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Times Cited By KSCI : 5  (Citation Analysis)
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