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http://dx.doi.org/10.5187/JAST.2010.52.3.175

Differential Proteome Expression of in vitro Proliferating Hanwoo Stromal Vascular Cells from Omental, Subcutaneous and Intramuscular Depots in Response to Hormone Deprivation and IGF-1, Estradiol-17β Addition  

Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
Kim, Seong-Kon (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
Park, Min-Ah (Department of Agricultural Science, Korea National Open University)
Kwon, Seulemina (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
Chang, Jong-Soo (Department of Agricultural Science, Korea National Open University)
Yoon, Du-Hak (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
Kim, Tae-Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of Animal Science and Technology / v.52, no.3, 2010 , pp. 175-186 More about this Journal
Abstract
The aim of this study was to analyze the proteome expressions of proliferating stromal vascular cells from Hanwoo omental, subcutaneous and intramuscular depots subjected to hormone deprivation and IGF-1, Estradiol-$17{\beta}$ addition. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further, to analyze the effect of insulin like growth factor (IGF-1) and $17\beta$-Estradiol (E2), cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or E2 (10 nM). The results showed that hormone deprivation had a negative impact on proliferation among the cells from all depots without any growth difference. On comparison of proliferation levels, higher levels were observed in cells that were grown in 10% FBS than in 10% CD-FBS alone or with IGF-1/E2. Proteome expression from preadipocytes grown in hormone deprivation conditions were compared by 2D-DIGE and MALDIToF/ToF. A total of twelve different proteins were found to be differentially expressed under hormone deprivation conditions. Further, our proteomic analysis with DIGE under IGF-1 and E2 addition revealed four proteins with differential expression levels. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to their effect in positive or negative regulation on proliferation.
Keywords
Stromal vascular cells; Preadipocytes; Proliferation; IGF-1; $17{\beta}$-Estradiol);
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