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http://dx.doi.org/10.5187/JAST.2008.50.6.783

Analysis of Differential Expression of Cytochrome P450 Aromatase(Cyp19) in The Efferent Ductules and The Epididymis of Male Rats During Postnatal Development  

Kim, Ju-Young (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
Seo, Hee-Jung (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
Kim, Ok-Soon (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
Kim, Byung-Joon (Departments of Biochemistry Internal Medicine, Eulji University School of Medicine)
Lee, Seong-Kyu (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
Baik, Haing-Woon (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
Lee, Ki-Ho (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
Publication Information
Journal of Animal Science and Technology / v.50, no.6, 2008 , pp. 783-792 More about this Journal
Abstract
The present study was performed to determine expression of cytochrome P450 aromatase(Cyp19) in the efferent ductules(EDs) and the epididymis of male rat reproductive tract at different postnatal ages. Total RNAs isolated were reverse-transcribed, and cDNAs were utilized for real-time PCR analysis. In the EDs, the Cyp19 transcript was expressed at all prepubertal ages with the highest level at 14 days of age, but not at 90 days of age. Expression of Cyp19 mRNA in the epididymis was found at all age groups, except 7 days of age. Distinct expression patterns of Cyp19 transcript were shown in each segment of the epididymis. These results indicate that expression of Cyp19 gene in the excurrent duct of male reproductive tract is differentially regulated in age-dependent and segment-specific manners.
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