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http://dx.doi.org/10.5187/JAST.2008.50.5.667

Effect of Dietary Lactobacillus on Growth Performance, Intestinal Microflora, Development of Ileal Villi, and Intestinal Mucosa in Broiler Chickens  

Kim, Sang-Ho (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Kim, Dong-Wook (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Park, Su-Young (Nonghyup Feed Inc., Korea)
Kim, Ji-Hyuk (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Kang, Geun-Ho (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Kang, Hwan-Ku (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Yu, Dong-Jo (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Na, Jae-Cheon (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Lee, Sang-Jin (Poultry Science Division, National Institute of Animal Science, R.D.A, Korea)
Publication Information
Journal of Animal Science and Technology / v.50, no.5, 2008 , pp. 667-676 More about this Journal
Abstract
This experiment was conducted to investigate the effects of dietary supplementation of various Lactobacilli strains on growth performance, intestinal microflora, ileal villi development and ileal mucosal surface of broiler chickens. Six hundred 1-d-old male chicks(Avian) were randomly divided into five groups of four replicates, each replicates containing 30 birds and fed corn-soybean meal diets containing Latobaillus were isolated from cecum of chichens at 107cfu/g diet for five weeks. The treatments were control(antibiotics-free diet), Lactobacillus crispatus avibro1(LCB), Lactobacillus reuteri avibro2(LRB), Lactobacillus crispatus avihen1(LCH) or Lactobacillus vaginalis avihen2(LVH). The body weight gain in groups fed Lactobacillus significantly improved as compared to those of control(P<0.05). Feed intake was not statistically different among the groups. The number of Lactobacillus in ileum and cecum of chicks fed various Lactobacillus tended to be increased or significantly increased as compared to those of the control(P<0.05), but there was difference by age of chicks and species of Lactobacillus. The number of yeast was significantly increased in cecum and ileum at three weeks old chicks fed Lactobacilli compared with the control(P<0.05). The anaerobes’ number of ileum and cecum tended to increase or significantly increased in Lactobacillus treatments compared with the control(P<0.05). The ileal villi length extended greatly at three weeks of age in groups fed Lactobacillus compared with the control(P<0.05). The length of ileal villi in chicks fed Lactobacillus was continuously increased up to five weeks of age, but did not increased in the control(P<0.05). Lactobacillus was found on ileal mucosal surface. And ileal mucosal surface was maintained better with Latobacillus feeding. These results suggest the possibility that cecal Lactobacillus of chickens could be used as probiotics by improving the growth performance and promoting development of ileal villi in broiler chicks.
Keywords
Lactobacillus; Broiler; Growth performance; Intestinal microflora; Ileal Villi; Intestinal mucosa
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1 Hill, R. P. H., Cowley, H. M. and Andremont, A. 1990. Influence of colonizing microflora on the mucin histochemistry of the neonatal mouse colon. Histochem J. 22:102-105   DOI
2 Impey, C. S. and Mead, G. C. 1989. Fate of Salmonellas in the alimentary tract of chicks pretreated with a mature caecal microflora to increase colonization resistance. J. Appl. Bacteriol. 66:469   DOI
3 Jin, L. Z., Ho, Y. W., Abdullah, N., Ali, M. A. and Jalaudin, S. 1998. Growth performance intestinal microbial populations and serum cholesterol of broilers fed diets containing Lactobacillus cultures. Poult. Sci. 77:1259-1265   DOI
4 Maiolino, R., Fioretti, A., Menna, L. F. and Meo, C. 1992. Research on the efficiency of probiotics in diets for broiler chickens. Nutrition Abstracts and Reviews Series B. 62:482
5 Cera, K. R., Mahan, D. C., Cross, R. F., Reinhart, G. A. and Whitmoyer, R. E. 1988. Effect of age weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine. J. Anim. Sci. 66:574   DOI
6 Duncan, D. B. 1955. Multiple range and multiple F test. Biometric. 11:1-42   DOI   ScienceOn
7 Jin L. Z., Ho, Y. W., Abdullah, N. and Jalaudin, S. 1996. Influence of dried bacillus subtillis and Lactobacilli cultures on intestinal microflora and performance in broilers. Asian-Australian J. Anim. Sci. 9:397-403   DOI
8 Lev, M. and Forbes, M. 1959. Growth response to dietary penicillin of germfree chicks and of chicks with a defined intestinal flora. Br. J. Nutr. 13:78-84   DOI   ScienceOn
9 Mohan, B., Kadirvel, R., Natarajan, A. and Bhaskaran, M. 1996. Effect of probiotic supplementation on growth nitrogen utilization and serum cholesterol in broilers. Br. Poult. Sci. 37:395-401   DOI   ScienceOn
10 NRC. 1994. Nutrient requirement of poultry. National Research Council National Academy of Science Washington DC
11 Fuller, R. and Turvey, A. 1971. Bacteria associated with the intestinal wall of the fowl Gallus domesticus. J. Appl. Bacteriol. 34:617-622   DOI
12 Nurmi, E. and Rantala, M. 1973. New aspects of Salmonella infection in broiler production. Nature. 241:210-211   DOI   ScienceOn
13 Prins, R. A. 1977. Biochemical activities of gut micro-organisms. In: Clarke, R. T. J. and Bauchop, T. eds. Microbial Ecology of the Gut. p73-183. London. Academic press
14 Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378   DOI
15 Fuller, R. 1992. Probiotics The scientific basis. Chapman and Hall. London
16 Watkins, B. A. and Kratzer, F. H. 1983. Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks. Poult. Sci. 62:2088-2094   DOI   ScienceOn
17 Yamaguchi, K., Isshiki, Y., Znou, Z. X. and Nakahiro, Y. 1990. Scanning and transmission electron microscopical observations of bacteria adhering to ileal epithelial cells in growing broiler and White Leghorn chickens. Br. Poult. Sci. 31:129-137   DOI   ScienceOn
18 Yeo, J. and Kim, K. 1997 Effect of feeding diets containing an antibiotic, a probiotic, or Yucca extract on growth and intestinal urease activity in broiler chicks. Poult. Sci. 76:381-385   DOI
19 Fuller, R. 1973. Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl. J. Appl. Bacteriol. 36:131-139   DOI
20 Dunham, H. J., William, C., Edens, F. W. Casas, I. A. and Dobrogosz, W. J. 1993. Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys. Poult. Sci. 72(Suppl 1):103(abstract)
21 Adler H. E. and DaMass, A. J. 1980. Effect of ingested Lactobacilli on Salmonella infantis and Escherichia coli and on intestinal flora pasted vents and chick growth. Avian Dis. 24:868-878   DOI   ScienceOn
22 Fuller, R. 1977. The importance of Lactobacilli in maintaining normal microbial balance in crop. Br. Poult. Sci. 18:85-94   DOI   ScienceOn
23 Hampson, D. J. 1986 Alterations in piglet small intestinal structure at weaning. Res. Vet. Sci. 40:32
24 Stavric S., Cleeson, T. M., Blanchfield, B. and Pivnick, H. 1987. Role of adhering microflora in competitive exclusion of Salmonella from young chicks. J. Food Protect. 50:928   DOI
25 Sakata, T. 1987. Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine: a possible explanation for trophic effects of fermentable fiber gut microbes and luminal trophic factors. Br. J. Nutr. 58:95   DOI   ScienceOn
26 SAS. 2002 SAS User's guide, Statistical Analysis System Inst. Inc Cary NC
27 Smith, M. W. 1985. Expression of digestive and absorptive function in differentiating enterocytes. Annu. Rev. Physiol. 47:247   DOI   ScienceOn
28 Watkins, B. A., Miller, B. F. and Neil, D. H. 1982. In vivo effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks. Poult. Sci. 61:1298-1308   DOI   ScienceOn