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http://dx.doi.org/10.5187/JAST.2008.50.4.573

Erythrocyte Manganese Superoxide Dismutase Activity Indicates Training Intensity for Racing Horses  

Choi, Jun-Young (Department of Animal Life Sciences, Laboratory of Nutrition and Biotechnology, Konkuk University)
Park, In-Kyung (Department of Animal Life Sciences, Laboratory of Nutrition and Biotechnology, Konkuk University)
Im, Jin-Taek (Department of Animal Life Sciences, Laboratory of Nutrition and Biotechnology, Konkuk University)
Koh, Tae-Song (Department of Animal Life Sciences, Laboratory of Nutrition and Biotechnology, Konkuk University)
Publication Information
Journal of Animal Science and Technology / v.50, no.4, 2008 , pp. 573-580 More about this Journal
Abstract
This study was aimed to investigate that training of horses is related with the activity of superoxide dismutase(SOD) in erythrocyte of racing horses. The SOD activity was assayed from erythrocyte of six Thoroubred horses having final stage of training, about 21 month-old, 474~509 kg body weight for race trainig. During 7 weeks of training period from 24th Sep. to 6th Nov, horses were bled very carefully 4 times at 1st Oct, 16th Oct, 30th Oct. and 6th Nov. As the training period passed, erythrocyte of the horses have gradually increased the MnSOD activity(p<0.05) and lowered the CuZnSOD activity. The plasma ceruloplasmin and peroxidase activities, and lactate levels were reduced gradually while peroxide and glucose levels gradually increased. The calculated oxygen consumption(Eaton, 1995) for training of horses were linearly related with the MnSOD activity(r=0.650, n=32) but negatively with CuZnSOD activity in erythrocyte and lactate levels(r=－349, n=32) in plasma. Also, peroxide levels in plasma of horses had positive relation with the MnSOD activity in erythrocyte(r=0.616, n=48). In conclusions, as the training is progressed, the raised MnSOD activity in erythrocytes and peroxide levels in plasma indicated balances between oxidant and antioxidants for the protection from ROS during race of horses. The results showed that the MnSOD activity in erythrocyte and peroxide levels in plasma may be used as marker for the intensity of training racing horses.
Keywords
Manganese superoxide dismutase (MnSOD); Plasma peroxide; Training horses
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