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http://dx.doi.org/10.5187/JAST.2008.50.4.445

The Amount of Telomeric DNA and Telomerase Activity on Cattle Cells  

Choi, Duk-Soon (Department of Animal Science and Biotechnology, Jinju National University)
Cho, Chang-Yeon (Animal Genetic Resources Station, National Institute of Animal Science)
Sohn, Sea-Hwan (Department of Animal Science and Biotechnology, Jinju National University)
Publication Information
Journal of Animal Science and Technology / v.50, no.4, 2008 , pp. 445-456 More about this Journal
Abstract
Telomeres consist of TTAGGG tandem repeated DNA sequences with specific proteins and locate at chromosome ends. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Telomerase is a ribonucleoprotein which has a template for the synthesis of telomeric DNA. This study was carried out to analyze the amount of telomeric DNA and telomerase activity in cattle cells. Analysis of the quantity of telomere in lymphocytes was done at different ages, sex and among Korean cattle and Holstein breeds. The telomerase activity was also analyzed in liver, brain, heart, kidney, and testis tissues of fetal calf and of 18 month old cattle. The amount of telomeres in lymphocytes and other tissue cells was analyzed by Quantitative-Fluorescence in situ Hybridization (Q-FISH) technique using a telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol assay (TRAP). The amount of telomeric DNA on the lymphocytes during the whole life span was decreased along with age. Quantity of telomeres in Korean cattle was significantly higher than that in Holstein breed. The amount of telomeric DNA in males was significantly higher than that in females. Telomerase activity was up-regulated in most bovine tissues during fetal stage, but was down-regulated in most tissues at mature 18 month age except the testis cells. This study indicates that the amount of telomeres and telomerase activity of cells can be used as an age marker or/and a physiological marker of cattle.
Keywords
Telomere; Telomerase; Q-FISH; TRAP; Cattle
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