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http://dx.doi.org/10.5187/JAST.2007.49.5.621

Isolation and Characteristics of Bacteriocin-producing Bacteria from the Intestine of Duck for Probiotics  

Shin, M.S. (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.)
Han, S.K. (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Ji, A.R. (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.)
Ham, M.R. (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.)
Kim, K.S. (Korea Bio Science Research Institute of Organic Bio Tech Co. Ltd.)
Lee, W.K. (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Animal Science and Technology / v.49, no.5, 2007 , pp. 621-632 More about this Journal
Abstract
The aim of this study was to isolate and characterize bacteriocin-producing bacteria from the intestine of duck to use as probiotics for livestock. A total of 416 strains were isolated from the small intestine and cecum of ducks and 13 isolates were finally selected after determinging inhibitory activity against pathogenic indicators by spot-on-lawn method. The selected strains were identified as Lactobacillus salivarius JWS 58, Lactobacillus plantarum JWS 1354, Pediococcus pentosaceus JWS 939, 7 strains of enterococci, and 3 strains of Escherichia coli. Lact. salivarius JWS 58, Ent. faecium JWS 833, and Ped. pentosaceus JWS 939 showed a strong inhibitory activity against Listeria monocytogenes. E. coli JWS 108 inhibited the growth of E. coli and Staphylococcus aureus. Lact. salivarius JWS 58 strain survived almost 50% in pH 2.5 phosphate buffer for 2 hr. Ped. pentosaceus JWS 939 and Lact. plantarum JWS 1354 showed strong amylolytic activity. These results suggest that a combination of bacteriocins or multispecies probiotics of the selected strains has a strong potential of alternative to antibiotics in livestock production.
Keywords
Bacteriocin; Probiotics; Duck; Livestock; Antibiotics;
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