[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5187/JAST.2007.49.3.309

Expression of Monocarboxylate Transporters(MCTs) and Basigin and Estrogen Receptor α(ERα)-Mediated Regulation of MCT1 Expression in The Efferent Ductules of Male Reproductive Tract During Postnatal Development

Lee, Ki-Ho (Department of Biochemistry-Molecular Biology and Center of Antiaging, Eulji University)
Choi, I.H. (School of Biotechnology, Yeungnam University)
Chung, C.S. (Department of Animal Science, Chungbuk National University)
Chang, J.S. (Department of Agricultural Sciences, Korea National Open University)

Publication Information

Journal of Animal Science and Technology / v.49, no.3, 2007 , pp. 309-320 More about this Journal

Abstract

The purpose of the present study was to determine if monocarboxylate transporter(MCT) isoforms and Basigin(Bsg) are expressed in the efferent ductules(ED) and if MCT1 expression is under estrogen receptor(ER)α-regulation in the ED of male reproductive tract. The presence of MCT isoforms and Bsg mRNAs was detected by real-time polymerization chain reaction(PCR), and ERα-mediated regulation of MCT1 expression in the ED was indirectly determined by immuno- histochemistry. Current study found differential expression of MCT isoforms(MCT1, 2, 3, 4, and 8) and Bsg mRNAs in rat ED according to postnatal ages. In addition, comparison of MCT1 expression in the ED between wild type and ERα knockout mice at different postnatal ages showed basolateral localization of MCT1 in ciliated cells of the ED and, in part, ERα- mediated regulation of MCT1 expression. It is suggested that MCTs would play a role in regulation of function of the ED.

Keywords

Efferent ductules; Monocarboxylate transporter; Basigin; Estrogen receptor $\alpha$ ; Estrogen;

Citations & Related Records

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