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http://dx.doi.org/10.5187/JAST.2006.48.6.805

Development of In vitro Fertilized Bovine Embryos in Medium Supplemented with Matrigel  

김동훈 (Animal Biotechnology Division, National Livestock Research Institute)
김세웅 (Animal Biotechnology Division, National Livestock Research Institute)
이민정 (Animal Biotechnology Division, National Livestock Research Institute)
황인선 (Animal Biotechnology Division, National Livestock Research Institute)
배성훈 (Animal Biotechnology Division, National Livestock Research Institute)
양병철 (Animal Biotechnology Division, National Livestock Research Institute)
임기순 (Animal Biotechnology Division, National Livestock Research Institute)
성환후 (Animal Biotechnology Division, National Livestock Research Institute)
양보석 (Animal Biotechnology Division, National Livestock Research Institute)
Publication Information
Journal of Animal Science and Technology / v.48, no.6, 2006 , pp. 805-812 More about this Journal
Abstract
The purpose of this study was to investigate the effect of Matrigel on the development of bovine embryos after in vitro fertilization. Bovine embryos were cultured in Ⅰ) SOF+ 0.8% BSA(SOF-B), Ⅱ) SOF+ 0.8% BSA plus 0.8% Matrigel(SOF-M) and III) SOF+0.8% BSA and 10% FBS(SOF-BF). The addition of Matrigel appeared not to increase the proportion of blastocysts (SOF-B, 26.6%; SOF-M, 28.2%; SOF-BF, 26.2%). However, the proportion of hatched blastocysts were significantly increased(P<0.05) by Matrigel(SOF-B, 23.7%; SOF-M, 48.7%; SOF-BF, 18.5%). The means of cell number blastocyst was not significantly different among the treatment groups(SOF-B, 172.7±35.5; SOF-M, 175.1±37.4; SOF-BF, 172.8±38.1). The proportion of apoptotic cells in blastocyst was also found to be not significant among the treatment groups(SOF-B, 3.6±3.2%; SOF-M, 4.3±2.6%; SOF-BF, 4.9±4.3%). In this experiment, Matrigel appeared to support embryonic hatching of bovine embryos. Results suggest that Matrigel, as extracellular matrix components, may be another avenue for formulating more physiological culture system in serum-free culture.
Keywords
Matrigel; Bovine; In vitro fertilization; Hatched blastocyst;
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