Browse > Article
http://dx.doi.org/10.5187/JAST.2006.48.6.785

Effect of Storage Times on Sperm Function, Sperm Chromatin Structure Assay (SCSA) and Correlations Between Fertility and SCSA in Boars  

유재원 (BET Research Institute, Chung-Ang University)
이주형 (Department of Animal Science & Technology)
김인철 (Department of Animal Science & Technology)
이일주 (Darby Genetics Inc)
강 권 (Darby Genetics Inc)
민동수 (Darby Genetics Inc)
윤희진 (Darby Genetics Inc)
윤종택 (Hankyong National University)
방명걸 (BET Research Institute, Chung-Ang University)
류범용 (BET Research Institute, Chung-Ang University)
정영채 (BET Research Institute, Chung-Ang University)
김창근 (BET Research Institute, Chung-Ang University)
Publication Information
Journal of Animal Science and Technology / v.48, no.6, 2006 , pp. 785-796 More about this Journal
Abstract
This study was designed to evaluate the changes in sperm motility, viability, HOST(hypo-osmotic swelling test), IVP(in vitro penetration), SCSA(sperm chromatin structure assay) during storage of liquid semen collected from boars with different farrowing rates using AI, and to find the relationship between boar fertility through AI and sperm diagnostic parameters during semen storage. The results of HOST were significantly decreased according to the increasing of in semen storage days and the results of IVP were significantly decreased at 3 days of semen storage (P<0.05). The %Red was significantly different among the >80%, 70󰠏80% and <70% farrowing rate group at semen storage day 6(P<0.05). The correlation coefficients between the %Red and farrowing rate were increased according to the semen storage. In conclusion, these results suggest that the sperm parameters evaluated in these studies may be useful indicators to predict the fertility of AI and evaluate the semen quality in boars.
Keywords
SCSA; Fertility;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ahmadi, A. and Ng, S. C. 1999. Fertilizing ability of DNA-damaged spermatozoa. J. Exp. Zool., 284:696-704   DOI   ScienceOn
2 Al-Qarawi, A., Abdel-Rahman, H. A., El-Mougy, S. A. and El-Belely, M. S. 2002. Use of a new computerized system for evaluation of spermatozoal motility and velocity characteristics in relation to fertility levels in dromedary bulls. Anim. Reprod. Sci., 74:1-9   DOI   ScienceOn
3 Ellington, J. E., Evenson, D. P., Fleming, J. E., Brisbois, R. S., Hiss, G. A. and Broder, S. J. 1998. Coculture of human sperm with bovine oviduct epithelial cells decreases sperm chromatin structural changes seen during culture in media alone. Fertil. Steril., 69:643-649   DOI   ScienceOn
4 Evenson, D. and Zbigniew Darzynkiewicz. 1990. Acridine orange induced precipitation of mouse testicular sperm cell DNA reveals new patterns of chromatin structure. Exp. Cell Res., 187:328-334   DOI   ScienceOn
5 Evenson, D. and Jost, L. 1994. Sperm chromatin structure assay : DNA denaturability. Methods Cell Biol., 42:159-176   DOI
6 Evenson, D. P., Larson, K. L. and Jost, L. K. 2002. Sperm chromatin structure assay : its clinical use for detecting sperm DNA fragmentation in male infertility and comparison with other techniques. J. Androl., 23:25-43   DOI
7 Ivanova, M. and Mollova, M. 1993. Zona-penetration in vitro test for evaluating boar sperm fertility. Theriogenology, 40:397-410   DOI   ScienceOn
8 Angelopoulos, T., Moshel, Y. A., Macanas, Lu L., Grifo, J. A. and Krey, L. C. 1998. Simultaneous assessment of sperm chromatin condensation and morphology before and after separation procedures : effect on the clinical outcome after in vitro fertilization. Fertil. Steril., 69:740-747   DOI   ScienceOn
9 Jeyendran, R. S., Van der Venn, H. H., Pérez- Peláez, M., Crabo, B. G. and Zanevel, L. J. D. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70:219-228   DOI   ScienceOn
10 Kirk, E. S., Squires, E. L. and Graham, J. K. 2005. Comparison of in vitro laboratory analyses with the fertility of cryopreserved stallion spermatozoa. Theriogenology, 64:1422-1439   DOI   ScienceOn
11 Berger, T., Anderson, D. L. and Penedo, M. C. T. 1996. Porcine sperm fertilizing potential in relationship to sperm functional capacities. Anim. Reprod. Sci., 44:231-239   DOI   ScienceOn
12 Bochenek, M., Smorg, Z. and Pilch, J. 2001. Sperm chromatin structure assay of bulls qualified for artificial insemination. Theriogenology, 56:557- 567   DOI   ScienceOn
13 Bordignon, V. and Smith, L. C. 1999. Ultraviolet- irradiated spermatozoa activate oocytes but arrest preimplantation development after fertilization and nuclear transplantation in cattle. Biol. Reprod., 61: 1513-1520   DOI   ScienceOn
14 Drevius, L. O. and Erikson, H. 1996. Osmotic swelling of mammalian spermatozoa. Exp. Cell Res., 42:136-156
15 Xu, Y. E., Wang Y. I., Lin, N., Zhang, J. W. and Qian S. Z. 1995. Subcapsular intratesticular assay: a preliminary screening method for male antifertility drugs. Int. J. Androl., 1:53-57.
16 Fraser, L. and Strzezek, J. 2004. The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5 degrees C and 16 degrees C. Folia. Histochem. Cytobio., 142:49-55
17 Xu, X., Pommier, S., Arbov, T., Hutchings, B., Sotto, W. and Foxcroft, G. R. 1998. In vitro maturation and fertilization techniques for assessment of semen quality and boar fertility. J. Anim. Sci., 76:3079-3089
18 Dadoune, J. P. 2003. Expression of mammalian spermatozoal nucleoproteins. Microsc. Res. Tech., 61:56-75   DOI   ScienceOn
19 Fatehi, A. N., Bevers, M. M., Schoevers, E., Roelen, B. A., Colenbrander, J. and Gadella, B. 2006. DNA damage in bovine sperm cells does not block fertilization but induces apoptosis after the first cleavages. J. Androl., 142:49-55
20 Gadea, J., Matas, C. and Lucas, X. 1998. Prediction of porcine semen fertility by homologous in vitro penetration(hIVP) assay. Anim. Reprod. Sci., 54: 95-108   DOI   ScienceOn
21 Gadea, J. and Matas, C. 2000. Sperm factors related to in vitro penetration of porcine oocytes. Theriogenology, 54:1343-1357   DOI   ScienceOn
22 Garner, D. L. and Johnson, L. A. 1995. Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol. Reprod., 53:276-284   DOI   ScienceOn
23 Hammitt, D. G., Martin, P. A. and Callanan, T. 1989. Correlations between heterospermic fertility and assays of porcine seminal quality before and after cryopreservation. Theriogenology, 32:385-399   DOI   ScienceOn
24 Harrison, R. A. P. 1997. Sperm plasma membrane characteristics and Boar semen fertility. J. Reprod. Fertil. Suppl., 52:271-283   DOI   ScienceOn
25 Love, C. C., Brinsko, S. P., Rigby, S. L., Thompson, J. A., Blanchard, T. L. and Varner, D. D. 2005. Relationship of seminal plasma level and extender type to sperm motility and DNA integrity. Theriogenology, 63:1584-1591   DOI   ScienceOn
26 Love, C. C., Thompson, J. A., Lowry, V. K. and Varner, D. D. 2002. Effect of storage time and temperature on stallion sperm DNA and fertility. Theriogenology, 57:1135-1142   DOI   ScienceOn
27 Sotolongo, B., Lino, E. and Ward, W. S. 2003. Ability of hamster spermatozoa to digest their own DNA. Biol. Reprod., 69:2029-2035   DOI   ScienceOn
28 Evenson, D. P., Jost, L. K., Baer, R. K., Turner, T. W. and Schrader, S. M. 1991. Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay. Reprod. Toxicol., 5:115-125   DOI   ScienceOn
29 Martinez, A. G., Matos, D. G., Furnus, C. C. and Brogliatti, G. M. 1998. In vitro evaluation and pregnancy rates after vitrification of in vitro produced bovine embryos. Theriogenology, 50:757-767   DOI   ScienceOn
30 Pérez-Llano, B., Lorenzo, J. L., Yenes, P., Trejo, A. and García-Casado, P. 2001. A short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology, 56:387-398   DOI   ScienceOn
31 Spano, M., Kolstad, H. A., Larsen, S. B., Cordelli, E., Leter, G. and Giwercman, A. 1998. The applicability of the flow cytometric sperm structure chromatin assay to epidemiological studies. Hum. Reprod., 13:2495-2505   DOI   ScienceOn
32 Vazquez-Levin, M. H., Notrica, J. A. and Polak, de Fried E. 1997. Male immunologic infertility : sperm performance on in vitro fertilization. Fertil. Steril., 68:675-681   DOI   ScienceOn
33 Martinez, E. A., Vazquez, J. M., Matas, C., Gadea, J., Alonso, M. I. and Roca, J. 1996. Oocyte penetration by fresh or stored diluted boar spermatozoa before and after in vitro capacitation treatments. Biol. Reprod., 55:134-140   DOI   ScienceOn
34 Vazquez, M. E., Verez, J. R., Stern, J. J., Gutierrez Najar, A. and Asch, R. H. 1998. Elevated basal estradiol levels have no negative prognosis in young women undergoing ART cycles. Gynecol. Endocrinol., 12:155-159   DOI
35 Evenson, D. P., Darzynkiewicz, Z. and Melamed, M. R. 1980. Relation of mammalian sperm chromatin heterogeneity to fertility. Science, 210:1131-1133   DOI
36 Boe-Hansen, Gry B., Annette K. Ersboll, Torben Greve and Preben Christensen. 2005. Increasing storage time of extended boar semen reduces sperm DNA integrity. Theriogenology, 63:2006-2019   DOI   ScienceOn
37 Benchaib, M., Braun, V., Lornage, J., Hadj, S., Salle, B. and Lejeune, H. 2003. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum. Reprod., 18: 1023-1028   DOI   ScienceOn
38 Ellington, J. E., Evenson, D. P., Wright, R. W., Jones, A. E., Schneider, C. S. and Fleming, J. E. 1999. Higher quality human sperm in a sample selectively attach to oviduct (fallopian tube) epithelial cell in vitro. Fertil. Steril., 71:924-929   DOI   ScienceOn
39 Berger, T. and Parker, K. 1989. Modification of the zona free hamster ova bioassay ofboa sperm fertility and correlation with in vivo fertility. Gamete Res., 22:385-397   DOI
40 Gil, M. A., Roca, J., Cremades, T., Hernández, M., Vázquez, J. M., Rodríguez-Martínez, H. and Martínez, E. A. 2005. Does multivariate analysis of post-thaw sperm characteristics accurately estimate in vitro fertility of boar individual ejaculates. Theriogenology, 64:305-316   DOI   ScienceOn
41 Van der Ven HH, Jeyendran, R. S., Al-Hasani, S., Perez-Pelaez, M., Diedrich, K. and Zaneveld, L. J. 1986. Correlation between human sperm swelling in hypoosmotic medium (hypoosmotic swelling test) and in vitro fertilization. J. Androl., 7:190-196   DOI