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http://dx.doi.org/10.5187/JAST.2004.46.6.947

Association Between the Polymorphism on Intron 5 of the Lipoprotein Lipase Gene and Carcass Traits in Hanwoo (Korean cattle)  

Lee, H.J. (National Livestock Research Institute, RDA, Department of Genetic Engineering, Sungkyunkwan University, Genetic Engineering Laboratory)
Lee, S.H. (National Livestock Research Institute, RDA)
Cho, Y.M. (National Livestock Research Institute, RDA)
Yoon, H.B. (National Livestock Research Institute, RDA)
Jeon, B. K. (National Livestock Research Institute, RDA)
Oh, S.J. (National Livestock Research Institute, RDA)
Kwon, M.S. (Department of Genetic Engineering, Sungkyunkwan University, Genetic Engineering Laboratory)
Yoon, D.H. (National Livestock Research Institute, RDA)
Publication Information
Journal of Animal Science and Technology / v.46, no.6, 2004 , pp. 947-956 More about this Journal
Abstract
The primary role of lipoprotein lipase(LPL) is the hydrolysis of triglycerides(TG) from the core of triglyceride-rich lipoproteins such as chylomicrons and very low density lipoproteins in plasma. Fatty acids liberated by LPL on capillary endothelial surfaces are available for tissues as energy sources especially in muscles or for storage in the form of TG in adipose tissues. Therefore, as the candidate gene related to the carcass traits of the beef cattle, we have directly sequenced the exon 5${\sim}$exon 6 region in the bovine LPL gene for discovery of single nucleotide polymorphism(SNP) with 24 unrelated Hanwoo(Korean cattle). Novel eight sequence variants were detected: three loci on exon 5, three on intron 5 and two on exon 6. All SNPs identified were strongly linked each other, and one hundred twenty eight Hanwoo samples were genotyped one SNP on intron 5 using PCR-restriction fragment length polymorphism method by digestion with Hae III restriction enzyme. The allele frequency of the polymorphism was 0.76 and 0.24. The effects of this polymorphism on the breeding values of the carcass weight, loin muscle area, back fat thickness and marbling score were analyzed using least square methods of SAS GLM. The marbling score of BB genotype was significantly higher than those of AA and AB genotypes(P<0.05). This result indicates that this polymorphism may be associated with the variation of marbling score. Further study is warranted to investigate the phenotypic association in Hanwoo.
Keywords
Lipoprotein lipase; Hanwoo; PCR-RFLP; Carcass traits;
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