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http://dx.doi.org/10.5187/JAST.2004.46.5.735

Application of Breed-specific DNA Markers for the use of Identifying Major Pure Pig Breeds Maintained in Korea  

Seo, B.Y. (Division of Applied Life Science, Gyeongsang National University)
Kim, J.H. (Division of Applied Life Science, Gyeongsang National University)
Park, E.W. (National Livestock Research Institute, R. D. A.)
Lim, H.T. (Division of Applied Life Science, Gyeongsang National University)
Cho, I.C. (National Institute of Subtropical Agriculture, R. D. A.)
Kim, B.W. (Division of Applied Life Science, Gyeongsang National University)
Oh, S.J. (National Livestock Research Institute, R. D. A.)
Cheong, I.C. (National Livestock Research Institute, R. D. A.)
Lee, J.G. (Division of Applied Life Science, Gyeongsang National University)
Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.5, 2004 , pp. 735-742 More about this Journal
Abstract
This study was conducted for the identification of pure Landrace, Large White and Duroc breeds which are mainly maintained in Korea using DNA markers. We used known KIT and MC1R mutations, which were related coat color in pigs, and pig mitochondrial DNA variations. The KIT mutation was used to distinguish white and colored animals. Duroc breed could be discriminated from other colored breeds using the MC1R mutation N121D. Discriminating Landrace and Large White was possible using the l l-bp duplication of D-Ioop region and alternative initiation codon of ND2. In conclusion, identification of Landrace, Large White and Duroc breeds was might be possible using the procedure designed in this study.
Keywords
Breed identification; Pig; MC1R; KIT; D-Ioop; ND2;
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1 Avise, J. C. 1986. Mitochondrial DNA and the evolutionary genetics of higher animals. Philos. Trans. R. Soc. Lond. B 312:325-342.   DOI   ScienceOn
2 Bowling, A. T., Valle, A D. and Bowling, M. 2000. A pedigree-based study of mitochondrial D-loop DNA sequence variation among arabian horses. Anim. Genet. 31:1-7.
3 Brown, W. M., George, M. and Wilson, A. C. 1979. Rapid evolution of animal mitochondrial DNA Proc. Nat!. Acad. Sci. 76:1967-1971.   DOI   ScienceOn
4 Lin, C. S., Sun, Y. L. and Liu, C. Y. 1999. Complete nucleotide sequence of pig(Sus scrofa) mitochondrial genome and dating evolutionary divergence within Artiodactyla. Gene 236: 107-114.   DOI   ScienceOn
5 Marklund, S., Kijas, 1., Martinez, H. R, Ronnstrand, L., Funa, K., Moller, M., Lange, D., Lilja, I. E. and Andersson, L. 1998. Molecular Basis for the Dominant White Phenotype in the Domestic Pig. Genome Res. 8:826-833.
6 Pielberg, G., Olsson, c., Syvanen, A C. and Andersson, L. 2002. Unexpectedly High Allelic Diversity at the KIT Locus Causing Dominant White Color in the Domestic Pig. Genetics 160: 305-31I.
7 Ursing, B. M. and Amason, U. 1998. The complete mitochondrial DNA sequence of the pig (Sus scrofa). 1. Mol. Evol. 47:302-306.
8 Giles, R E., Blanc, H., Cann, H. M. and Wallace, D. C. 1980. Maternal inheritance of human mitochondrial DNA Proc. Natl. Acad. Sci. 77:6715-6719.   DOI   ScienceOn
9 Evert, R E., Rothuizen, J. and van Oost, B. A 2000. Identification of a premature stop codon in the melanocyte-stimulating hormone receptor gene (MCIR) in labrador and golden retrievers with yellow coat color. Anim. Genet. 31:194-199.
10 Giuffra, E., Evans, G., Tornsten, A, Wales, R, Day, A, Looft, H., Plastow, G. and Andersson, L. 1999. The Belt mutation in pigs is an allele at the Dominant white(I/KIT) locus. Mamm. Genome 10(12): 1132-1136.
11 Giuffra, E., Tomsten, A, Marklund, S., Rudloff, E. B., Chardon, P., Kijas, J. M. H., Anderson, S. I., Alan, L. and Archibald, L. A 2002. A Large duplication associated with dominant white color in pigs originated by homologous recombination between LINE elements flanking KIT. Mamm. Genome 13:569-557.
12 Irwin, D. M., Kocher, T. D. and Wilson, A C. 1991. Evolution of the cytochrome b gene of mammals. J. Mol. Evol. 32:128-144.
13 Kavar, T., Habe, F., Brem, G. and Dove, P. 1999. Mitochondrial D-Ioop sequence variation among the 16 maternal lines of the Lipizzan horse breed. Anim. Genet. 30:102-108.
14 Kijas, J. M. H., Wales, R, Tornsten, A, Chardon, P., Moller, M. and Andersson, L. 1998. Melanocortin Receptor I(MCIR) Mutations and Coat Color in Pigs. Genetics 150:1177-1185.
15 조인철. 2004. 돼지 mtDNA와 MCIR 유전자를 이용한 계통유전학적 유연관계: 제주지역 재래돼지와 한반도 야생멧돼지를 중심으로 경상대학교 박사학위논문.
16 Kim, K. I., Lee, J. H., Li, K., Zhang, Y. P., Lee, S. S., Gongora, J. and Moran, C. 2002. Phylogenetic relationships of Asian and European pig breeds determined by mitochondrial DNA D-Ioop sequence polymorphism. Anim. Genet. 33: 19-25   DOI   ScienceOn
17 Wilson, A. C., Cann, L., Carr, S. M., George, M. and Gyllensten, U. B. 1985. Mitochondrial DNA and two perspectives on evolutionary genetics. Biol. J. Linn. Soc. 26:375-400.   DOI
18 조인철, 정용환, 정진관, 성필남, 오운용, 고문석, 김병우, 이정규, 전진태. 2004. 돼지 Melanocortin Receptor 1(MCIR) 대립유전자 3의 신규 유전변이 탐색. 한국동물자원과학회지. 46:1-6.
19 한상현, 조인철, 최유림, 이종언, 고문석, 김재화, 서보영, 이정규, 전진태. 2004. 돼지 5품종에 있어서 mtDNA ND2 유전자의 선택적 개시코존의 특성과 빈도. 한국동물자원과학회지 투고중.