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http://dx.doi.org/10.5187/JAST.2004.46.1.077

Purification of Angiogenin from Bovine Milk  

Nam, M.S. (Lab. of Milk Food Biochemistry, Division of Animal Science & Resources, College of Agriculture and Life Sciences, Chungnam National University)
Bae, H.C. (Lab. of Milk Food Biochemistry, Division of Animal Science & Resources, College of Agriculture and Life Sciences, Chungnam National University)
Park, C.S. (Research Center for Transgenic Cloned Pigs, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.1, 2004 , pp. 77-82 More about this Journal
Abstract
This study was carried out to establish the purification protocol of angiogenin(ANG) from bovine milk. The purification of ANG from bovine milk was performed by using cation chromatography, high-performance liquid chromatography and gel-filtration. We obtained the ANG protein have the molecular weight of about 14 kD by SDS-PAGE analysis. This protein was confirmed as ANG by Nlb-terminal sequence analysis of the first 15 amino acids. Identified amino acids revealed the protein to be identical to that previously reported for bovine ANG.
Keywords
Angiogenin; Amino acid sequence; N-terminal amino acid sequence;
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