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http://dx.doi.org/10.5187/JAST.2004.46.1.023

Molecular Cloning and mRNA Expression of the Bovine Peroxisome Proliperator Receptor Gamma(PPARγ)  

Jeoung, Y.H. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Lee, S.M. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Park, H.Y. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Yoon, D.H. (National Liverstock Research Institute)
Choi, J.G. (National Liverstock Research Institute, Daekwanryeong Branch)
Moon, S.J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Kang, M.J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.1, 2004 , pp. 23-30 More about this Journal
Abstract
The peroxisome proliferator-activated receptor $\gamma$(PPAR$\gamma$), a member of the steroid/thyroid nuclear hormone receptor suferfamily of ligand-activated transcription factor, is an important regulator of adipocyte gene expression and differentiation. In this studies, we report the identification, characterization, and expression of a Hanwoo PPAR$\gamma$ gene. The PPAR$\gamma$ cDNA sequence of the Hanwoo show strong conservation with the corresponding sequences reported in other species except of three amino acid sequences. The distribution of PPAR$\gamma$ mRNA in various tissues of Korean cattle aged 12 months were investigated using Northern Blot analysis. The highest expression was detected in adipose tissue, more lower expression was detected in colon, small intestine, kidney, lung, while expression was not detected in brain, heart. PPAR$\gamma$ expression was higher in adipose tissue of Korean cattle when aged 30 months than aged 12 months. These results indicated PPAR$\gamma$, regulator adipocyte gene expression and differentiation, related on adipose differentiation in Korean native cattle(HANWOO).
Keywords
Adipocyte differentiation; PPAR$\gamma$; Hanwoo;
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