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http://dx.doi.org/10.5187/JAST.2002.44.6.677

Expression Patterns of the Differentially Expressed Genes During Growth Stages of Hanwoo(Korean Cattle)  

Jang, Y.S. (National Livestock Research Institute, R.D.A.)
Yoon, D.H. (National Livestock Research Institute, R.D.A.)
Kim, T.H. (National Livestock Research Institute, R.D.A.)
Cheong, I.C. (National Livestock Research Institute, R.D.A.)
Jo, J.K. (Department of Animal Science, Kyungpook National University)
Publication Information
Journal of Animal Science and Technology / v.44, no.6, 2002 , pp. 677-684 More about this Journal
Abstract
We have investigated the expression patterns of candidates for growth stage specifically expressed genes. The expression patterns of the EPV20, aldolase A, Translationally Controlled Tumor Protein (TCTP) and Adipocyte Differentiation Related Protein (ADRP) were examined by semiquantitative RT-PCR and northern blot analysis in skeletal muscle tissues of Hanwoo, especially in the longissimus dorsi at various growth stages. The EPV20 mRNA was expressed in longissimus dorsi tissue of Hanwoo, but there was no difference of expression levels during growth stages. Though the aldolase A gene was reported to be muscle-specific and regulated at developmental stages, the expression levels of aldolase A mRNA in the longissimus dorsi tissues showed little differences at various growth stages. The expression levels of TCTP which was reported as growth-related protein regulated at translation step were gradually increased during growth of Hanwoo. The expression levels of ADRP mRNA were rapidly increased at 24-month-old longissimus dorsi tissue of Hanwoo, and decreased at 30-month-old. Our data suggest that the ADRP gene show as growth-stage dependent expression and is related to fat deposition within muscular tissue.
Keywords
Hanwoo; Expression pattern; EPV 20; Aldolase A; TCTP; ADRP;
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