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http://dx.doi.org/10.5187/JAST.2002.44.2.181

Sequence and Genetic Variation of Mitochondrial DNA D-loop Region in Korean Cattle  

Chung, E.R. (Division of Applied Animal Sciences, Sangji University)
Kim, W.T. (Division of Applied Animal Resources, Kangwon National University)
Kim, Y.S. (Division of Applied Animal Resources, Kangwon National University)
Lee, J.K. (Division of Applied Animal Resources, Kangwon National University)
Han, S.K. (College of Animal Husbandry, Konkuk University)
Publication Information
Journal of Animal Science and Technology / v.44, no.2, 2002 , pp. 181-190 More about this Journal
Abstract
This study was performed to determine sequences of the mt DNA D-loop region, including $tRNA^{Pro}$ and $tRNA^{Pre}$ and to analysis sequence variation polymorphism in Korean cattle. The resulting sequencies were compared with previously published sequences for other cattle breeds(GenBank J01394). The PCR was used to amplify an 1142bp between nucleotides 15061 and 404 within the D-loop region of mt DNA using specific primers. Korean cattle showed 24 polymorphic sites by nucleotide substitutions and insertions of single base pairs. About 50% of polymorphic sites were found in positions 16042 to 16122 with the most variable region. Among these polymorphic sites, variations at 16055, 16230 and 16260 bp were detected as new sequence variants in Korean cattle. These specific polymorphic sites have not been reported in the Japanese black cattle and European cattle. Therefore, mt DNA variants in the D-loop region may be used as genetic markers for specifying Korean cattle. The frequencies of positions 169, 16302, 16093, 16042, 16119 with a high level of sequence polymorphism were 0.81, 0.56, 0.56, 0.50 and 0.43, respectively. In comparison of genetic distances, Korean cattle showed the more closely to European cattle as Bos taurus than Bos indicus such as African and India breeds. In conclusion, these mt DNA sequence polymorphisms in the D-loop region for Korean cattle may be useful for the analysis of cytoplasmic genetic variation and associations with economic important traits and genetic analysis of maternal lineage.
Keywords
mt DNA D-loop region; Sequence variation; Korean cattle;
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