Browse > Article
http://dx.doi.org/10.12749/RDB.2014.38.4.165

Expression Patterns of Apoptosis, Adhesion and Immune related Proteins in Uterine Endometrium with Normal Ovarian Follicles and Ovarian Cyst in Hanwoo  

Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University)
Lee, Yong-Hee (Department of Biotechnology, College of Engineering, Daegu University)
Chae, Sung-Kyu (Department of Biotechnology, College of Engineering, Daegu University)
Min, Sung-Hun (Department of Biotechnology, College of Engineering, Daegu University)
Ahn, Jae-Hyun (Department of Biotechnology, College of Engineering, Daegu University)
Do, Geon-Yeop (Department of Biotechnology, College of Engineering, Daegu University)
Kim, Byung Oh (School of Food Science & Biotechnology, College of Agriculture & Life Sciences, Kyungpook National University)
Park, Humdai (Department of Biotechnology, College of Engineering, Daegu University)
Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
Publication Information
Abstract
Cows may suffer impaired ovarian function, often accompanied by reduced conception rates and increased embryonic loss. Cystic ovarian disease (COD) is one of the most frequently diagnosed gynecological findings in dairy cattle. It causes temporary infertility and is likely to affect reproduction as well as production parameters in cattle. Therefore, the purpose of this study was to determine the expression patterns of apoptosis (Bcl-2, Bax), implantation (E-cadherin) and immune related proteins (TNF-${\alpha}$, IL-10) in uterine endometrium of Hanwoo (Korean native cattle) with ovarian cyst and normal ovarian follicles. In the Western blot analysis, the expression of anti-apoptotic Bcl-2 protein was significantly higher in endometrium with normal ovarian follicles, whereas expression of pro-apoptotic Bax protein was significantly lower. Also, the expressions of E-cadherin and TNF-${\alpha}$ proteins were significantly higher in uterine endometrium with normal ovarian follicles. On the other hand, the expression of IL-10 protein was significantly lower in uterine endometrium with normal ovarian follicles. Taken together, our results provided that the expressions of apoptosis, adhesion and immune related proteins in uterine endometrium with ovarian cyst were showed the aberrant patterns, and we suggest that different expression changes of these proteins may be affect to pregnancy ability of cattle.
Keywords
Protein expression; Endometrium; Ovarian cyst; Cattle;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Achache H, Revel A (2006): Endometrial receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 12:731-746.   DOI   ScienceOn
2 Alfaro NS, Salvetti NR, Velazquez MM, Stangaferro ML, Rey F, Ortega HH (2012): Steroid receptor mrna expression in the ovarian follicles of cows with cystic ovarian disease. Res Vet Sci 92:478-485.   DOI   ScienceOn
3 Aplin JD (2000): The cell biological basis of human implantation. Baillieres Best Pract Res Clin Obstet Gynaecol 14:757-764.   DOI   ScienceOn
4 Barth AI, Nathke IS, Nelson WJ (1997): Cadherins, catenins and Apc protein: Interplay between cytoskeletal complexes and signaling pathways. Curr Opin Cell Biol 9:683-690.   DOI   ScienceOn
5 Clark DA, Chaouat G, Arck PC, Mittruecker HW, Levy GA (1998): Cytokine-dependent abortion in cba x dba/2 mice is mediated by the procoagulant Fgl2 prothombinase. J Immunol 160:545-549.
6 Denker HW (1993): Implantation: A cell biological paradox. J Exp Zool 266:541-558.   DOI   ScienceOn
7 Harada T, Kaponis A, Iwabe T, Taniguchi F, Makrydimas G, Sofikitis N, Paschopoulos M, Paraskevaidis E, Terakawa N (2004): Apoptosis in human endometrium and endometriosis. Hum Reprod Update 10:29-38.   DOI   ScienceOn
8 Harada T, Taniguchi F, Izawa M, Ohama Y, Takenaka Y, Tagashira Y, Ikeda A, Watanabe A, Iwabe T, Terakawa N (2007): Apoptosis and endometriosis. Front Biosci 12:3140-3151.   DOI
9 Hong SO, Lee BS, Yang WI, Lee JS, Cha DH, Cho YS, Kim JY, Park KH, Cho DJ, Song CH (2000): BCL-2 and BAX expression in normal human endometrium. Kor J Fertil Steril 27:245-251.
10 Konno R, Yamakawa H, Utsunomiya H, Ito K, Sato S, Yajima A (2000): Expression of survivin and Bcl-2 in the normal human endometrium. Mol Hum Reprod 6:529-534.   DOI   ScienceOn
11 Krasnow JS, Tollerud DJ, Naus G, DeLoia JA (1996): Endometrial Th2 cytokine expression throughout the menstrual cycle and early pregnancy. Human Reproduction 8:1747-1754.
12 Moreno-Bueno G, Hardisson D, Sarrio D, Sanchez C, Cassia R, Prat J, Herman JG, Esteller M, Matias-Guiu X, Palacios J (2003): Abnormalities of Eand P-cadherin and catenin (beta-, gamma-catenin, and P120ctn) expression in endometrial cancer and endometrial atypical hyperplasia. J Pathol 199:471-478.   DOI   ScienceOn
13 Oltvai ZN, Milliman CL, Korsmeyer SJ (1993): Bcl-2 heterodimerizes in vivo with a conserved homolog, bax, that accelerates programmed cell death. Cell 74:609-619.   DOI   ScienceOn
14 Otsuki Y (2001): Apoptosis in human endometrium: Apoptotic detection methods and signaling. Med Electron Microsc 34:166-173.   DOI   ScienceOn
15 Pantaleo M, Piccinno M, Roncetti M, Mutinati M, Rizzo A, Sciorsci RL (2013): Evaluation of serum concentrations of interleukin (Il)-4, Il-10, and Il-12 during pregnancy in bitches. Theriogenology 79:970-973.   DOI   ScienceOn
16 Park AJ, Lee MK (2005): Peripheral Th1 and Th2 lymphocytes values by intracellular cytokine staining for interferon-${\gamma}$ and interleukin-4 in the patients with repeated spontaneous abortion. Korean J Lab Med 25:111-115.
17 Tao XJ, Tilly KI, Maravei DV, Shifren JL, Krajewski S, Reed JC Tilly JT, Isaacson KB (1997): Differential expression of members of the bcl-2 gene family in proliferative and secretory human endometrium: glandular epithelial cell apoptosis is associated with increased expression of bax. J Clin En docrinol Metab 82:2738-2746.
18 Peter AT (2004): An update on cystic ovarian degeneration in cattle. Reprod Dom Anim 39:1-7.   DOI   ScienceOn
19 Raghupathy R (1997): Th1-type immunity is incompatible with successful pregnancy. Immunol Today 18:478-482.   DOI   ScienceOn
20 Salvetti NR, Alfaro NS, Velazquez MM, Amweg AN, Matiller V, Diaz PU, Ortega HH (2012): Alteration in localization of steroid hormone receptors and coregulatory proteins in follicles from cows with induced ovarian follicular cysts. Reproduction 144:723-735.   DOI   ScienceOn
21 Vanholder T, Opsomer G, Kruif AD (2006): Aetiology and pathogenesis of cystic ovarian follicles in dairy cattle: A review. Reprod Nutr Dev 46:105-119.   DOI   ScienceOn
22 Vaskivuo TE, Stenback F, Karhumaa P, Risteli J, Dunkel L, Tapanainen JS (2000): Apoptosis and apoptosis-related proteins in human endometrium. Mol Cell Endocrinol 165:75-83.   DOI   ScienceOn
23 Velazquez MM, Salvetti NR, Amweg AN, Diaz PU, Matiller V, Ortega HH (2013): Changes in the expression of heat shock proteins in ovaries from bovines with cystic ovarian disease induced by acth. Res Vet Sci 95:1059-1067.   DOI   ScienceOn
24 Watanabe H, Kanzaki H, Narukawa S, Inoue T, Katsuragawa H, Kaneko Y, Mori T (1997): Bcl-2 and fas expression in eutopic and ectopic human endometrium during the menstrual cycle in relation to endometrial cell apoptosis. Am J Obstet Gynecol 176:360-368.   DOI   ScienceOn