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http://dx.doi.org/10.12749/RDB.2014.38.3.115

Effect of Humulus japonicus Extract on Sperm Motility, Fertilization Status and Subsequent Preimplantation Embryo Development in Cattle  

Min, Sung-Hun (Department of Biotechnology, College of Engineering, Daegu University)
Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University)
Do, Geon-Yeop (Department of Biotechnology, College of Engineering, Daegu University)
Lee, Yong-Hee (Department of Biotechnology, College of Engineering, Daegu University)
Ahn, Jae-Hyun (Department of Biotechnology, College of Engineering, Daegu University)
Chae, Sung-Kyu (Department of Biotechnology, College of Engineering, Daegu University)
Kim, Byung Oh (School of Food Science & Biotechnology, College of Agriculture & Life Sciences, Kyungpook National University)
Park, Humdai (Department of Biotechnology, College of Engineering, Daegu University)
Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
Publication Information
Reproductive and Developmental Biology / v.38, no.3, 2014 , pp. 115-121 More about this Journal
Abstract
Humulus japonicus is an ornamental plant in the Cannabaceae family. Although the mode of action of Humulus japonicus is not fully understood, a strong relationship was observed between anti-inflammatory and anticancer in some types of cells. Recent studies also have shown that Humulus japonicus possesses anti-inflammatory activities and may significantly improve antioxidant potential in Raw 264.7 macrophage cells. Thus, the aim of this study was evaluated the effect of Humulus japonicus extract on sperm motility and subsequent preimplantation developmental competence of the bovine embryos. After in vitro maturation, the oocytes with sperms were exposed in in vitro fertilization (IVF) medium supplemented with Humulus japonicus extract (0.01, 0.05, $0.1{\mu}g/mL$, respectively) for 1 day. In our results, exposure of IVF medium to Humulus japonicus extract did not affect sperm motility and percentage of penetrated oocytes but ROS intensity was significantly decreased by $0.01{\mu}g/mL$ compared with other groups (p< 0.05). Moreover, treatment with $0.01{\mu}g/mL$ of Humulus japonicus extract was higher the frequency of blastocyst formation than the any other groups (p<0.05). Otherwise, treatment with $0.01{\mu}g/mL$ of Humulus japonicus extract not increased the total cell number but reduced apoptotic-positive nuclei number. In conclusion, our results indicate that supplementation of Humulus japonicus extract in IVF medium may have important implications for improving early embryonic development in bovine embryos.
Keywords
Humulus japonicus; In vitro fertilization; ROS; Apoptosis; Cattle;
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