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http://dx.doi.org/10.12749/RDB.2013.37.2.57

Effects of Trichostatin A on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos  

Diao, Yun-Fei (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Kenji, Naruse (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Han, Rong-Xun (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Lin, Tao (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Oqani, Reza-K. (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Kang, Jung-Won (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Jin, Dong-Il (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Publication Information
Reproductive and Developmental Biology / v.37, no.2, 2013 , pp. 57-64 More about this Journal
Abstract
Developmental potential of cloned embryos is related closely to epigenetic modification of somatic cell genome. The present study was to investigate the effects of applying histone deacetylation inhibitor, trichostatin A (TSA) to activated porcine embryos on subsequent development of porcine parthenogenetic and nuclear transfer embryos. Electrically activated oocytes were treated with 5 nM TSA for different exposure times (0, 1, 2 and 4 hr) and then the activated embryos were cultured for 7 days. The reconstructed embryos were treated with different concentrations of 0, 5, 10 and 25 nM TSA for 1 hr. Also 5 nM TSA was tested with different exposure times of 0, 0.5, 1, 2 and 4 hr. And fetal fibroblast cells were treated with 50 nM TSA for 1, 2 or 4 hr and with 5 nM TSA for 1 hr. Cumulus-free oocytes were enucleated and reconstructed by TSA-treated donor cells and electrically fused and cultured for 6 days. In parthenogenetic activation experiments, 5 nM TSA treatment for 1 hr significantly improved the percentage of blastocyst developmental rates than the other groups. Total cell number of blastocysts in 1 hr group was significantly higher than other groups or control. Similarly, blastocyst developmental rates of porcine NT embryos following 5 nM TSA treatment for 1 hr were highest. And the reconstructed embryos from donor cells treated by 50 nM TSA for 1 hr improved the percentage of blastocyst developmental rates than the control group. In conclusion, TSA treatment could improve the subsequent blastocyst development of porcine parthenogenetic and nuclear transfer embryos.
Keywords
Porcine; reconstructed embryos; Trichostatin A; Parthenogenetic activation;
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