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http://dx.doi.org/10.12749/RDB.2013.37.1.17

Identification of Stage-specific Genes Related to Porcine Folliculogenesis  

Lee, Jae Hee (Stem Cell and Bioevaluation Laboratory, WCU Biomodulation Program, Seoul National University)
Lee, Seung Tae (Department of Animal Biotechnology, Kangwon National University)
Kim, Heebal (Department of Agricultural Biotechnology, Seoul National University)
Lim, Jeong Mook (Stem Cell and Bioevaluation Laboratory, WCU Biomodulation Program, Seoul National University)
Publication Information
Reproductive and Developmental Biology / v.37, no.1, 2013 , pp. 17-22 More about this Journal
Abstract
Although assisted reproductive technology is very useful to develop novel and therapeutic biomaterials for reproduction, research on molecular mechanism of folliculogenesis in pig is not clear. Therefore, the alteration of gene expression during follicular development in pigs was examined in this study. The expression of folliculogenesis-related genes was quantified in preantral ($250{\sim}300{\mu}m$) and antral (> $300{\mu}m$ in diameter) follicles, and overall gene expression was evaluated by a genome-wide microarray. The microarray results showed that 219 genes were differentially expressed, and of those, 10 and 22 known genes showed higher and less expression at the preantral stage than at antral stages, respectively. Among them, the expression of NR0B1, PPARG, GATA4, and ANXA2 genes related to folliculogenesis was validated by quantitative real-time PCR analysis. The expression of PPARG and GATA4 genes were increased at antral stages, but a significantly stage-specific increase (p<0.05) was only detected in annexin A2 (ANXA2) in antral-stage follicles. The expression of NR0B1 genes was increased at preantral stage and these patterns of gene expression were comparable to the results obtained by microarray analysis. We propose that the systematical regulation of genes supporting specific follicle stage should be employed for improved in-vitro folliculognesis.
Keywords
Antral follicles; Gene expression; Ovary; Preantral follicles;
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1 Banerjee J, Komar CM (2006): Effects of luteinizing hormone on peroxisome proliferator-activated receptor gamma in the rat ovary before and after the gonadotropin surge. Reproduction 131:93-101.   DOI   ScienceOn
2 Bocca SM, Billiar RB, Albrecht ED, Pepe GJ (2008): Oocytes of baboon fetal primordial ovarian follicles express estrogen receptor beta mRNA. Endocrine 33: 254-260.   DOI   ScienceOn
3 Bolstad BM, Irizarry RA, Astrand M, Speed TP (2003): A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19:185-193.   DOI   ScienceOn
4 Bonnet A, Lê Cao KA, Sancristobal M, Benne F, Robert- Granié C, Law-So G, Fabre S, Besse P, De Billy E, Quesnel H, Hatey F, Tosser-Klopp G (2008): In vivo gene expression in granulosa cells during pig terminal follicular development. Reproduction 136: 211-224.   DOI   ScienceOn
5 Chun SY, Bae HW, Kim WJ, Park JH, Hsu SY, Hsueh AJ (2001): Expression of messenger ribonucleic acid for the antiapoptosis gene P11 in the rat ovary: gonadotropin stimulation in granulosa cells of preovulatory follicles. Endocrinology 142:2311-2317.   DOI
6 Danielsen EM, Deurs BV, Hansen GH (2003): "Nonclassical" secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane. Biochemistry 42:14670-14676.   DOI   ScienceOn
7 Drummond AE (2006): The role of steroids in follicular growth. Reprod Biol Endocrinol 4:16.   DOI   ScienceOn
8 Duggavathi R, Volle DH, Mataki C, Antal MC, Messaddeq N, Auwerx J, Murphy BD, Schoonjans K (2008): Liver receptor homolog 1 is essential for ovulation. Genes Dev 22:1871-1876.   DOI   ScienceOn
9 Emans N, Gorvel JP, Walter C, Gerke V, Kellner R, Griffiths G, Gruenberg J (1993): Annexin II is a major component of fusogenic endosomal vesicles. J Cell Biol 120:1357-1369.   DOI
10 Eppig JJ (2001): Oocyte control of ovarian follicular development and function in mammals. Reproduction 122:829-838.   DOI   ScienceOn
11 Feldman AL, Costouros NG, Wang E, Qian M, Marincola FM, Alexander HR, Libutti SK (2002): Advantages of mRNA amplification for microarray analysis. Biotechniques 33: 906-912, 914.
12 Gautier L, Cope L, Bolstad BM, Irizarry RA (2004): Affy-analysis of Affymetrix GeneChip data at the probe level. Bioinformatics 20:307-315.   DOI   ScienceOn
13 Gillio-Meina C, Hui YY, LaVoie HA (2003): GATA- 4 and GATA-6 transcription factors: expression, immunohistochemical localization, and possible function in the porcine ovary. Biol Reprod 68:412-422.   DOI
14 Lovekamp-Swan T, Chaffin CL (2005): The peroxisome proliferator-activated receptor gamma ligand troglitazone induces apoptosis and p53 in rat granulosa cells. Mol Cell Endocrinol 233:15-24.   DOI   ScienceOn
15 Grindflek E, Sundvold H, Klungland H, Lien S (1998): Characterisation of porcine peroxisome proliferatoractivated receptors gamma 1 and gamma 2: detection of breed and age differences in gene expression. Biochem Biophys Res Commun 249:713-718.   DOI   ScienceOn
16 Hara S, Takahashi T, Amita M, Igarashi H, Tsutsumi S, Kurachi H (2011): Bezafibrate restores the inhibition of FSH-induced follicular development and steroidogenesis by tumor necrosis factor-alpha through peroxisome proliferator-activated receptor-gamma pathway in an in vitro mouse preantral follicle culture. Biol Reprod 85:895-906.   DOI   ScienceOn
17 Hashimoto S, Ohsumi K, Tsuji Y, Harauma N, Miyata Y, Fukuda A, Hosoi Y, Iritani A, Morimoto Y (2007): Growing porcine oocyte-granulosa cell complexes acquired meiotic competence during in vitro culture. J Reprod Dev 53:379-384.   DOI   ScienceOn
18 Mao J, Smith MF, Rucker EB, Wu GM, McCauley TC, Cantley TC, Prather RS, Didion BA, Day BN (2004): Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro. J Anim Sci 82:1967-1965.   DOI
19 Mao J, Wu G, Smith MF, McCauley TC, Cantley TC, Prather RS, Didion BA, Day BN (2002): Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol Reprod 67:1197-1203.   DOI   ScienceOn
20 Oh H-S, Nychka DW, Lee TCM (2007): The role of pseudo data for robust smoothing with application to wavelet regression. Biometrika 94:893-904.   DOI   ScienceOn
21 Wang J, Hu L, Hamilton SR, Coombes KR, Zhang W (2003): RNA amplification strategies for cDNA microarray experiments. Biotechniques 34:394-400.
22 Puskas LG, Zvara A, Hackler L Jr, Van Hummelen P (2002): RNA amplification results in reproducible microarray data with slight ratio bias. Biotechniques 32:1330-1334, 1336, 1338, 1340.
23 Salmon NA, Handyside AH, Joyce IM (2005): Expression of Sox8, Gata4, Wt1, Dax1, and Fog2 in the mouse ovarian follicle: implications for the regulation of Amh expression. Mol Reprod Dev 70: 271-277.   DOI   ScienceOn
24 Smyth GK (2004): Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3:Article3.
25 Wu MF, Huang WT, Tsay C, Hsu HF, Liu BT, Chiou CM, Yen SC, Cheng SP, Ju JC (2002): The stagedependent inhibitory effect of porcine follicular cells on the development of preantral follicles. Anim Reprod Sci 73:73-88.   DOI   ScienceOn
26 Yang KT, Lin CY, Huang HL, Liou JS, Chien CY, Wu CP, Huang CW, Ou BR, Chen CF, Lee YP, Lin C, Tang PC, Lee WC, Ding ST, Cheng WT, Huang MC (2008): Expressed transcripts associated with high rates of egg production in chicken ovarian follicles. Mol Cell Probes 22:47-54.   DOI   ScienceOn
27 Yang FM, Pan CT, Tsai HM, Chiu TW, Wu ML, Hu MC (2009): Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells. FEBS J 276:425-436.   DOI