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Effect of Porcine Follicular Fluid on Donor Cell Characteristics and Quality of Porcine Cloned Blastocysts  

Kwon, Dae-Jin (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Lee, Jeong Woong (Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Sung-Soo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Chang, Won-Kyong (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Publication Information
Reproductive and Developmental Biology / v.36, no.4, 2012 , pp. 249-254 More about this Journal
Abstract
This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF (pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group (p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and quality of the cloned blastocyst.
Keywords
Porcine follicular fluid; Amino acids; Cell cycle; Cloned embryo development;
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