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Effect of Bcl-2 Inhibitor Treatment on Embryo Developmental Competence, Apoptosis and ER-stress in Pigs  

Hong, Joo-Hee (Department of Biotechnology, College of Engineering, Daegu University)
Min, Sung-Hun (Department of Biotechnology, College of Engineering, Daegu University)
Lee, Enok (Department of Biotechnology, College of Engineering, Daegu University)
Son, Hyeong-Hoon (Department of Biotechnology, College of Engineering, Daegu University)
Yeon, Ji-Yeong (Department of Biotechnology, College of Engineering, Daegu University)
Park, Humdai (Department of Biotechnology, College of Engineering, Daegu University)
Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
Publication Information
Reproductive and Developmental Biology / v.36, no.3, 2012 , pp. 167-172 More about this Journal
Abstract
The key regulators of apoptosis are the interacting protein of the Bcl-2 family. Bcl-2, an important member of this family, blocks cytochrome C release by sequestering pro-apoptotic BH3-only proteins such as Bid, Bad, Bax and Bim. The pro-survival family members (Bcl-2, Bcl-XL, Bcl-W) are critical for cell survival, since loss of any of them causes cell death in certain cell type. However, its role during early porcine embryonic development is not sufficient. In this study, we traced the effects of Bcl-2 inhibitor, ABT-737, on early porcine embryonic development. We also investigated several indicators of developmental potential, including gene expression (apoptosis-related genes) and apoptosis, which are affected by ABT-737. Porcine embryos were cultured in the PZM-3 medium with or without ABT-737 for 6 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without ABT-737 ($14.7{\pm}3.0$ vs $30.3{\pm}4.8%$, p<0.05). TUNEL assay showed that the number of containing fragmented DNA at the blastocyst stage increased in the ABT-737 treated group compared with control (4.7 vs 3.7, p<0.05). The mRNA expression of the pro-apoptotic gene Bax increased in ABT-737 treated group (p<0.05), whereas expressions of the anti-apoptotic Bcl-2 family members (Bcl-2, Bcl-XL, Bcl-W) decreased (p<0.05). Also, expressions of the ER stress indicator genes (GRP78, XBP-1 and sXBP-1) increased in ABT-737 treated group (p<0.05). In conclusion, Bcl-2 is closely associated with of apoptosis- and ER stress-related genes expressions and developmental potential in pig embryos.
Keywords
Bcl-2 inhibitor; Developmental ability; Apoptosis; ER-stress; Pig;
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