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Polyclonal Antibody to a 37-kDa Recombinant Protein Derived from Bovine $20{\alpha}$-Hydroxysteroid Dehydrogenase  

Naidansuren, Purevjargal (Animal Biotechnology, Graduate School of Bio & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Min, Kwan-Sik (Animal Biotechnology, Graduate School of Bio & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Publication Information
Reproductive and Developmental Biology / v.36, no.2, 2012 , pp. 109-114 More about this Journal
Abstract
We prepared the polyclonal antibody anti-$20{\alpha}$-hydroxysteroid dehydrogenase (anti-$20{\alpha}$-HSD) against the recombinant full-length protein bovine $20{\alpha}$-HSD in Escherichia coli. The specificity of anti-$20{\alpha}$-HSD was demonstrated using Chinese hamster ovary (CHO) cells transfected with recombinant bovine $20{\alpha}$-HSD and bovine placental tissues. According to western blot analysis, anti-$20{\alpha}$-HSD specifically recognizes the 37-kDa protein bovine $20{\alpha}$-HSD. The protein is not present in untransfected CHO cells. Anti-$20{\alpha}$-HSD also recognizes a specific protein in the ovaries and placenta of other animals. Immunostaining was used to detect expression of bovine $20{\alpha}$-HSD protein in the cultured luteal cells during the estrous cycle later.
Keywords
Polyclonal antibody; Bovine; $20{\alpha}$-HSD; Ovary; Placenta;
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