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Effect of Magnetized Water on Cryopreservation in Bovine Spermatozoa  

Seo, Gi-Beom (College of Animal Life Sciences, Kangwon National University)
Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University)
Lee, Kyung-Jin (College of Animal Life Sciences, Kangwon National University)
Yu, Han-Jun (College of Animal Life Sciences, Kangwon National University)
Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Lee, Seung-Hwan (National Institute of Animal Science, Hanwoo Ex)
Lee, Jin-Woo (ROHAS TECH)
Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.35, no.3, 2011 , pp. 363-367 More about this Journal
Abstract
The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle. Before cryopreservation, without egg-yolk Triladyl$^{(R)}$ solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for S min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen-thawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.
Keywords
Korean native cattle; Spermatozoa; Cryopreservation; Magnetized water;
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