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Effect of Storage in Different Commercial Semen Extenders on the Motility, Viability and Fertility In Vitro of Boar Spermatozoa  

Sa, Soo-Jin (National Institute of Animal Science, RDA)
Kim, Myung-Jick (National Institute of Animal Science, RDA)
Cho, Kyu-Ho (National Institute of Animal Science, RDA)
Kim, Du-Wan (National Institute of Animal Science, RDA)
So, Kyoung-Min (National Institute of Animal Science, RDA)
Chung, Ki-Hwa (Gyeongnam National University of Science and Technology)
Son, Jung-Ho (Noah Biotech. Inc.)
Kim, In-Cheul (National Institute of Animal Science, RDA)
Publication Information
Reproductive and Developmental Biology / v.35, no.3, 2011 , pp. 203-207 More about this Journal
Abstract
The objective of this study was to determine the effect of semen extenders on the motility, viability and fertility in vitro of spermatozoa during storage of fresh boar semen diluted in different commercial extenders used for pig artificial insemination (AI). In this experiment, semen were diluted in Androhep plus, Beltsville Thawing Solution (BTS), Modena, Seminark and Vitasem LD. Five ejaculates were collected from three Duroc boars and sub-samples were diluted ($30{\times}10^6$ spermatozoa/ml) in different extenders. Semen was stored at $170^{\circ}C$ for 10 days. Sperm motility and viability was assessed using Computer-Assisted Semen Analysis (CASA) and flow-cytometry on 1, 3, 5 and 10 day post collection The motility of spermatozoa stored in different extenders was gradually decreased by increasing the duration of storage of semen. However, there was not significant1y different in the sperm motility and viability among other extenders. On the other hand, the in vitro-matured oocytes were fertilized and cultured in vitro to assess the fertility of boar spermatozoa stored for 3 and 10 days in different extenders. The percentage of morula and blastocyst were taken as indicators of fertility in vitro of spermatozoa. Therefore, there were no differences in the rate of embryos developed to the molular and blastocyst stage. There were no differences in the motility and fertility in vitro among 5 kinds of commercial boar semen extenders.
Keywords
Boar semen extender; Motility; Viability; Fertility in vitro; Spermatozoa;
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1 Britt JH, Almond GW, Flowers WL (1999): Diseases of the reproductive system. In: Straw B, D'Allaire S, Mengeling W, Taylor D (eds), Diseases of Swine, 8th edn. Blackwell Science Ltd, Ames, IA, p. 905.
2 Dube C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey JL (2004): Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation. Theriogenology 62: 874-886.   DOI   ScienceOn
3 Huo LJ, Ma XH, Yang ZM (2002): Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long term storage. Theriogenology 58:1349-1360.   DOI   ScienceOn
4 Johnson LA, Weize KF, Fiser P, Maxwell WMC (2000): Storage of boar semen. Anim Reprod Sci 62:143- 172.   DOI   ScienceOn
5 Kommisrud E, Paulenz H, Sehested E, Grevle IS (2002): Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta Veterinaria Scandinavica 43:49- 55.   DOI
6 김인철 (2005): 돼지 인공수정 현황과 발전방안. 농촌진흥청 축산연구소. pp 8-25.
7 Boe-Hansen GB, Ersboll AK, Greve T, Christensen P (2005): Increasing storage time of extended boar semen reduces sperm DNA integrity. Theriogenology 63:2006-2019.   DOI   ScienceOn
8 Marco DA, Juan B, Fernado S, Ignacio C, Anders J, Margarteta W, Magnus A, Heriberto R (2006): Effect of storage in short- and long-term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa. International Journal of Andrology 29:543-552.   DOI   ScienceOn
9 Maxwell WMC, Johnson LA (1997): Membrane status of boar spermatozoa after cooling or cryopreservation. Theriogenology 48:209-219.   DOI   ScienceOn
10 Vyt P, Maes D, Dejonckheere E, Castryck F, Van Soom A (2004): Comparative study on five different commercial extenders for boar semen. Reproduction in Domestic Animals 39:8-12.   DOI   ScienceOn