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Effects of Cryo-extenders for Spermatozoa Sorted by Percoll on In Vitro Fertility of in Miniature Pigs  

Lee, Sang-Hee (College of Animal Life Science, Kangwon National University)
Yoo, Han-Jun (College of Animal Life Science, Kangwon National University)
Lee, Yong-Seung (College of Animal Life Science, Kangwon National University)
Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
Km, Dae-Young (Division Biological Science, Gachon University of Medical and Science)
Park, Choon-Keun (College of Animal Life Science, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.35, no.1, 2011 , pp. 85-91 More about this Journal
Abstract
The objective of this study was to evaluated the efficiency on sperm cryosurvival and ability of in vitro fertilization using Triladyl and Lactose Egg-Yolk(LEY) as extenders for cryopreservation of separated sperm by 65% percoll in miniature pig. Sperm viability was measured with SYBR-14/PI double stained sperm by flow cytometry. Ability on embryo cleavage rate and blastocyst development were observed by in vitro fertilization after frozen-thawing of sperm separated by 65% percoll. The experimental groups were designed that separated sperm by 65% percoll with Triladyl (ST) or LEY(SL) and unseparated sperm with Triladyl(UT) or LEY(UL) for cryopreservation. As a results, the viability was significantly(p<0.05) higher in ST(55.1%), SL(63.1%), UL(58.8%) than UT(38.2%) group. Sperm viability in SL(63.1%) group was significantly(p<0.05) higher than other experimental groups. On the other hand, embryo cleavage rate was significantly(p<0.05) higher in ST(79.1%), SL(83.2) than UT(74.1) and UL(75.7%) groups at 96h after in vitro fertilization. Blastocyst development was also significantly(p<0.05) higher in ST(21.5%), SL(20.9%) than UT(17.0%) and UL(18.8%) groups. In conclusion, cryopreservation of miniature boar sperm separated by 65% percoll were beneficial to viability and capacity on in vitro fertilization.
Keywords
Miniature pig's sperm; Cryopreservation; Extenders; Percoll; In vitro fertilization;
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Times Cited By KSCI : 5  (Citation Analysis)
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