Browse > Article

Effect of Estrogenic Xonibiotics on Boar Sperm Motility and Motion Kinematics  

Oh, Shin-Ae (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University)
Park, Yoo-Jin (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University)
Song, Won-Hee (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University)
Mohamed, El-Sayed A. (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University)
Pang, Myung-Geol (Department of Animal Science and Technology, School of Bioresource & Bioscience, Chung-Ang University)
Publication Information
Reproductive and Developmental Biology / v.35, no.1, 2011 , pp. 47-54 More about this Journal
Abstract
Endocrine disruptors bind to hormone receptors on sperm membrane, therefore spermatozoa are potentially a useful model for examining estrogenic activities of endocrine disruptors. The objective of this study was to compare the effects of two xenoestrogenic compounds [genistein (Gen) and 4-tert-octylphenol (OP)] to those of two steroids [estrogen ($E_2$) and progesterone ($P_4$)] on boar sperm % motility and motion kinematics of in vitro. Porcine spermatozoa were incubated with various concentrations ($0.001{\sim}100\;{\mu}M$) of each chemical for 15 or 30 min, and then assessed % motility and sperm motion kinematics using computer assisted sperm analyzer (CASA). Each chemical decreased sperm % motility, and OP decreased VSL and VAP compared with untreated control(p<0.05). $E_2$ stimulated the motion kinematic changes except VCL. Moreover, Gen had effects on VCL and VAP alterations after 30 min incubation. In summary, since all chemicals studied effectively altered sperm % motility and motion kinematics, it was concluded that porcine spermatozoa could be a useful model for in vitro screening of potential endocrine disruptors.
Keywords
Boar spermatozoa; Endocrine disruptors; Xenobiotics; Motility; Motion kinematics;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Couse JF, Korach KS (1999): Estrogen receptor null mice: what have we learned and where will they lead us? Endocr Rev 20:358-417.   DOI   ScienceOn
2 Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J (2010): Effect of estrogens on boar sperm capacitation in vitro. Reprod BioI Endocrinol 13:87-97.
3 Akingbemi BT, Hardy MP (2001): Oestrogenic and antiandrogenic chemicals in the environment: effects on male reproductive health. Ann Med 33:391-403.   DOI   ScienceOn
4 Aydogan M, Barlas N (2006): Effect of maternal 4-tert-octylphenol exposure on the reproductive tract of male rats and adulthood. Reprod Toxicology 22:455-460.   DOI   ScienceOn
5 Adeoya-Osiguwa SA, Markoulaki S, Pocock V, Milligan SR, Fraser LR (2003): 17beta-estradiol and environmental estrogens significantly affect mammalian sperm function. Hum Reprod 18:100-107.   DOI   ScienceOn
6 Watson CE, Gauthier SY, Davies PL (1999): Structure and expression of the highly repetitive hustine H1- related sperm chromatin proteins from winter flounder. J Bio Chem 262:258-267.
7 Yanagimachi R (1994): Mammalian fertilization. In: Knobil E, Neill JD, editors. The Physiology of Eproduction. New York: Raven Press. pp 189-317.
8 Tubbs C, Thomas P (2009): Progestin signaling through an olfactory G protein and membrane progestin receptor-alpha in Atlantic croaker sperm: potential role in induction of sperm hypermotility. Endocrinology. 150:473-484.
9 Uhler ML, Leung A, Chan SYW, Wang C (1992): Direct effects of progesterone and antiprogesterone on human sperm hyperactivated motility and acrosome reaction. FertiI Steril 58:1191-1198.
10 Stormshak F, Bishop CV (2008): Estrogen and progesterone signaling: genomic and nongenomic actions in domestic ruminants. J Anim Sci 86:299-315.   DOI
11 Watson CE, Gauthier SY, Davies PL (1999): Structure and expression of the highly repetitive hustine H1-related sperm chromatin proteins from winter flounder. J Bio Chem 262:258-267.
12 Sirivaidyapong S, Bevers MM, Gadella BM, Colenbrander B (2001): Induction of the acrosome reaction in dog sperm cells is dependent on epididymal maturation: the generation of a functional progesterone receptor is involved. Mol Reprod Dev 58:451-459.   DOI   ScienceOn
13 Skakkebaek NE, Rajpert-De Meyts E, Main KM (2001): Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Hum Reprod 16:972-978.   DOI
14 Sohoni P, Sumpter JP (1998): Several environmental oestrogens are also anti-androgens. J Endocrinol 158:327-339.   DOI   ScienceOn
15 Saberwal GS, Sharma MK, Balasinor N, Choudhary J, Juneja HS (2002): Estrogen receptor, calcium mobilization and rat sperm motility. Mol Cell Biochem 237:11-20.   DOI
16 Tubbs C, Thomas P (2008): Functional characteristics of membrane progestin receptor (mPR) subtypes: a review with new data showing mPR expression in seatrout sperm and its association with sperm motility. Steroids 73:935-941.   DOI   ScienceOn
17 Saberwal GS, Sharma MK, Balasinor N, Choudhary J, Juneja HS (2002): Estrogen receptor, calcium mobilization and rat sperm motility. Mol Cell Biochem 237:11-20.   DOI
18 Sharpe RM (2001): Hormones and testis development and the possible adverse effects of environmental chemicals. Toxicol Lett 120:221-232.   DOI   ScienceOn
19 Sirianni R, Chimento A, Ruggiero C, De Luca A, Lappano R, Ando S, Maggiolini M, Pezzi V (2008): The novel estrogen receptor, G protein-coupled receptor 30, mediates the proliferative effects induced by 17 beta-estradiol on mouse spermatogonial GC-1 cell line. Endocrinology 149:5043-51.   DOI   ScienceOn
20 Rago V, Aquila S, Panza R, Carpino A (2007): Cytochrome $P_{450}arom$, androgen and estrogen receptors in pig sperm. Reprod BioI Endocrinol 2007;5:23-28.   DOI
21 Rathi R, Colenbrander B, Stout TAE, Bevers MM, Gadella BM (2003): Progesterone induces acrosome reaction in stallion spermatozoa via a protein tyrosine kinase dependent pathway. Mol Reprod Dev 64:120-128.   DOI   ScienceOn
22 Rochira V, Granata AR, Madeo B, Zirilli L, Rossi G Carani C (2005): Estrogens in males: what have we learned in the last 10 years. Asian J Androl 7:3-20.   DOI   ScienceOn
23 Saaranen MJ, Calvo L, Dennison L, Banks S, Bustillon AD, Dorfmann M, Goldstein M, Thorsell L, Schulmann JD, Sherins RJ (1993): Acrosome reaction inducing activity in follicular fluids correlates with progesterone concentration but not with oocyte maturity or fertilizability. Hum Reprod 8:1448-1454.
24 Mohamed ES, Park YJ, Song WH, Shin DB, You YA, Ryu BY, Pang MG (2011): Xenoestrogenic compounds promote capacitation and an acrosome reaction in porcine sperm. Theriogenology 75(6):1161-9.   DOI   ScienceOn
25 Neild DN, Gadella BM, Aguero A, Stout TAE, Colenbrander B (2005): Capacitation, acrosome finction and chromatin structure in stallion sperm. Anim Reprod Sci 89:47-56.   DOI   ScienceOn
26 Luconi M, Muratori M, Forti G, Baldi E (1999): Identification and characterization of a novel functional estrogen receptor on human sperm membrane that interferes with progesterone effects. J Clin EndocrinoI Metab 84:1670-1678.   DOI   ScienceOn
27 Milligan SR, Kalita JC, Heyerick A, Rong H, De Cooman L, De Keukeleire D (1999): Identification of a potent phytoestrogen in hops (Humulus lupulus L.) and beer. J Clin Endocrinol Metab 83:2249-2252.
28 Milligan SR, Kalita JC, Pocock V, Van de Kauter V, Stevens JF, Deinzer ML, Rong H, De Keukeleire D (2000): The endocrine activities of 8-prenylnaringenin and related hop (Humulus lupulus L.) flavonoids. J Clin Endocrinol Metab 85:4912-4915.
29 Misao R, Niwa K, Morishita S, Fujimoto J, Nakanishi Y, Tamaya T (1997): Immunohistochemical detection of estrogen and progesterone receptors in spermatozoa of infertile men. Int J Fertil 42:421-425.
30 Kim JC, Li Y, Lee S, Yi YJ, Park CS, Woo SH (2008): Effects of cryopreservation on $Ca^{2+}$ signals induced by membrane depolarization, caffeine, thapsigargin and progesterone in boar spermatozoa. Mol Cells 26(6):558-565.
31 Kuiper GGJM, Carlsson B, Grandien K, Enmark E, Haggblad J, Nilsson S, Gustafsson JA (1998): Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology 138:863-870.
32 Libersky EA, Boatman DE (1995): Progesterone concentrations in serum, follicular fluid, and oviductal fluid of the golden hamster during periovulatory period. BioI Reprod 53:477-482.   DOI   ScienceOn
33 Luconi M, Bonaccorsi L, Forti G, Baldi E (2001): Effects of estrogenic compounds on human spermatozoa: evidence for interaction with a nongenomic receptor for estrogen on hmnan sperm membrane. Mol Cell Endocrinol 178:39-45.   DOI
34 Luconi M, Francavilla F, Porazzi I, Macerola B, Forti G, Baldi E (2004): Human spermatozoa as a model for studying membrane receptors mediating rapid nongenomic effects of progesterone and estrogens. Steroids 69:553-559.   DOI   ScienceOn
35 Dixon RA (2004): Phytoestrogens. Annu Rev Plant BioI. 55:225-261.   DOI   ScienceOn
36 Durkee TJ, Mueller M Zinaman M (1998): Identification of estrogen receptor protein and messenger ribonucleic acid in human spermatozoa. Am J Obstet GynecoI. 178:1288-1297.   DOI   ScienceOn
37 Durkee TJ, Mueller M, Zinaman M (1998): Identification of estrogen receptor protein and messenger ribonucleic acid in human spermatozoa. Am J Obstet Gynecol 178:1288-1297.   DOI   ScienceOn
38 Fraser LR, Beyret E, Milligan SR, Adeoya-Osiguwa SA (2006): Effects of estrogenic xenobiotics on human and mouse spermatozoa. Hum Reprod 21:1184-1193.   DOI   ScienceOn