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Expression Analysis of Matrix Metalloproteinases and Tissue Inhibitor of Matrix Metalloproteinases from In Vitro Maturation Oocytes Complexes in Porcine  

Kim, Sang-Hwan (Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University)
Kang, Hyun-Ah (Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University)
Kim, Dae-Seung (Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University)
Lee, Myeong-Seop (Medi Kinetics Co., Ltd.)
Seo, Kang-Suk (Department of Animal Science and Technology, Sunchon National University)
Min, Kwan-Sik (Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University)
Yoon, Jong-Taek (Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University)
Publication Information
Reproductive and Developmental Biology / v.34, no.1, 2010 , pp. 55-62 More about this Journal
Abstract
Matrix metalloproteinases (MMP) play important roles in extracellular matrix (ECM) remodeling during ovarian follicular development, oocytes development and ovulation. In an attempt to investigate the effect of MMP activation in development cumulus-oocytes complexes, we examined the localization and expression of MMP, and monitored MMP expression profile. Cumulus-oocytes complexes were collected and matured in vitro for 24 hr, 36 hr and 48 hr. A mRNA expression of MMP-2, MMP-9, TIMP-2 and TIMP-3 was detected in all culture medium regardless of CC, DC and CDCs. Activity of MMP-2 in the DC progressively was increased from 24 hr to 48 hr. But MMP-9 was not detected in all culture medium. The localization of MMP-2 was also measured by immunohistochemistry analysis. The MMP-2 and TIMP-2 was detected in cumulus cell and oocyte zone pellucida. Expression of MMP-2 protein in the COCs was progressively increased from 24 hr to 48 hr. However, MMP-9 protein was progressively decreased from 24 hr to 48 hr. And TIMP-2 protein was most highly expressed in the CDCs 36 hr. Expression of TIMP-3 protein in the CDCs was progressively increased from 24 hr to 48 hr. In conclusion, these results suggest that MMP-2 plays a role in maintaining normal maturation and development by controlling the ECM inhibitor concentration on cumulus cell and oocytes.
Keywords
MMP2; MMP9; TIMP2; TIMP3; Cumulus-oocytes complexes;
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