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The Comparison of Triladyl and LEY for Cryosurvival Improvement of Sperm Separated by Percoll in Miniature Pig  

Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University)
Yoo, Han-Jun (College of Animal Life Sciences, Kangwon National University)
Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University)
Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Kim, Dae-Young (Division Biological Science, Gachon University of Medical and Science)
Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.34, no.1, 2010 , pp. 41-46 More about this Journal
Abstract
The objective of this study was to evaluated the efficiency of sperm cryosurvival using each extenders Triladyl and LEY containing egg yolk to the cryopreservation of separated sperm by percoll in miniature pig. The ejaculated semen from miniature pig was separated by 65% percoll and un-separated sperm as a control before freezing. The freezing of diluted semen added with Triladyl containing egg yolk and LEY solution (solution I: 11% Lactose or Triladyl + egg yolk; solution II: solution I + glycerol + OEP). Analysis of sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CIC) the morphologic abnormality and hypoosmotic swelling test(HOST). The groups were designed that as separated sperm by Percoll with Triladyl(ST) or LEY(SL) for cryopreservation. And unseparated sperm with Triladyl(UT) or LEY(UL). As a results, the viability was higher significantly(p<0.05) in ST, SL, UT than UL extender. The morphologic abnormality was measured significantly (p<0.05) lower in ST than other extenders. The AR-patterned in CTC analysis was measured significantly(p<0.05) lower in SL and UL than other extenders. In conclusion, using Triladyl extender resulted in viability and morphology of separated sperm by percoll that were effective than using LEY extender, but it resulted in capacitation acrosome reaction was lower than using LEY extender.
Keywords
Miniature pig; Sperm; Cryopreservation; Extender; Percoll;
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Times Cited By KSCI : 3  (Citation Analysis)
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