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Studies on the Specific Gene Expression in Lung Tissue of the Cloned Hanwoo  

Kim, Sang-Hwan (The Animal Biotechnology, GSBIT, Hankyong National University)
Chung, Duck-Won (The Animal Biotechnology, GSBIT, Hankyong National University)
Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University)
Hwang, Sue-Yun (The Animal Biotechnology, GSBIT, Hankyong National University)
Min, Kwan-Sik (The Animal Biotechnology, GSBIT, Hankyong National University)
Yoon, Jong-Taek (The Animal Biotechnology, GSBIT, Hankyong National University)
Publication Information
Reproductive and Developmental Biology / v.33, no.1, 2009 , pp. 19-24 More about this Journal
Abstract
This study was conducted to investigate the specific expression genes in the cloned bovine tissues. Donor cells, cloned tissues were analysed by RAPD-RFLP method. The results were detected three genes (CH-U7B, CH-U7M and CH-U7P) in the cloned fetus. It was found a single copy genes by southern hybridization. Sequence analysis of CH-U7M gene was shown 99% homology to a previously reported EST from a cloned bovine fetus. The putative ORF was encode a protein of hydrophobicity index 0.03. Semi-quantitative RT-PCR by using the CH-LS001 specific primer was remarkably detected in the lung tissue of cloned fetus. Further investigation of these genes may provide one of the key information to explain the early death, abnormal fetus, large off-spring and the low pregnancy rate in the production of cloned bovine.
Keywords
Cloned Hanwoo; Specific gene expression; Lung;
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