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Efficient Gene Delivery into Hematopoietic Stem Cells by Intra-Bone Marrow Injection of Retrovirus  

Lee, Byun-Joo (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Lee, Yong-Soo (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Hye-Sun (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Yu-Kyung (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Kim, Jae-Hwan (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Park, Jin-Ki (Animal Biotechnology Division, National Livestock Research Institute)
Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research Institute)
Chang, Won-Kyong (Animal Biotechnology Division, National Livestock Research Institute)
Kim, Dong-Ku (Graduate School of Life Science and Biotechnology, College of Medicine, CHA Stem Cell Institute, Pochon CHA University)
Publication Information
Reproductive and Developmental Biology / v.32, no.1, 2008 , pp. 9-14 More about this Journal
Abstract
Efficient gene transfer into hematopoietic stem cells is a great tool for gene therapy of hematopoietic disease. Retrovirus have been extensively used for gene delivery and gene therapy. However, current in vitro gene transfer has some obstacles suck as induction of differentiation loss of self-renewal capacity, and down-regulation of homing efficiency for in vitro hematopoietic stem cells transplantation. To overcome these problems, we developed efficient in vitro retroviral transfer technique by direct intra-bone marrow injection (IBM). We identified effective retrovirus gene transfer in bone marrow hematopoietic cells in vitro. Two weeks after retrovirus transfer via IBM injection, we observed stable EGFP gene expression in bone marrow, lymph node, spleen, and liver cells. In addition, $6.4{\pm}2.7%$ of hematopoietic stem/progenitor cells were expressed EGFP transgene from flow cytometry analysis. Our results demonstrate that in vitro retrovirus gene transfer via IBM injection can provide a viable alternative to current or moo gene transfer approach.
Keywords
Hematopoietic stem cells; In vitro gene transfer; IBM injection; Retrovirus;
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