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The Expression of TGF-${\beta}_1$ Protein Level during Periparturient Periods in the Recipients Pregnant by SCNT Embryos  

Hwang, Seong-Soo (National Institute of Animal Science, RDA)
Chang, Yoo-Min (Chung-Ang University)
Ko, Yeoung-Gyu (National Institute of Animal Science, RDA)
Yang, Byong-Chul (National Institute of Animal Science, RDA)
Im, Gi-Sun (National Institute of Animal Science, RDA)
Kim, Myong-Jik (National Institute of Animal Science, RDA)
Min, Kwan-Sik (Hankyong University)
Yoon, Jong-Taek (Hankyong University)
Kim, Chang-Keun (Chung-Ang University)
Seong, Hwan-Hoo (National Institute of Animal Science, RDA)
Publication Information
Reproductive and Developmental Biology / v.32, no.1, 2008 , pp. 27-31 More about this Journal
Abstract
This study was performed to investigate the correlations between steroids and TGF-${\beta}_1$ levels and delayed parturition in SCNT clone calving. The recipients pregnant by AI were used as control (AI-R). All AI-R were labored by natural delivery (n=5, day $284{\pm}0.71$ of pregnancy). The recipients pregnant by SCNT embryo (SCNT-R) showing no signs of delivery about 10 days after expected date were operated by Caesarean section (n=5, day 292). The blood and placentome samples were obtained and weighed at parturition. The concentrations of plasma progesterone (P4) and Estradiol-$17{\beta}$ (E2) were measured by radioimmunoassay (RIA). The levels of plasma and placental TGF-${\beta}_1$ levels were examined by ELISA. The placentomes from SCNT-R were overweight (p<0.05) compared to those of AI-R. The plasma P4 (p<0.01) level in SCNT-R at parturition was significantly higher compared to that of AI-R. In contrast, the plasma E2 level in the SCNT-R was significantly lower compared to that of AI-R (p<0.05). The plasma and placental TGF-${\beta}_1$ protein levels in the SCNT-R were significantly higher than those of AI-R at parturition, respectively (p<0.01). Based on these results, aberrant expressions of steroid hormones and high levels of plasma and placental TGF-${\beta}_1$ protein at parturition may be one of the key indicators on delayed parturition of SCNT clone calving.
Keywords
SCNT; Progesterone; Estrogen; TGF-${\beta}_1$; Delayed parturition;
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