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Influences of LH, FSH, EGF and Cysteine on In Vitro Canine Oocyte Maturation  

Song, Hye-Jin (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Kang, Eun-Ju (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Ock, Sun-A (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Jeon, Byeong-Gyun (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Rho, Gyu-Jin (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Choe, Sang-Yong (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Reproductive and Developmental Biology / v.31, no.3, 2007 , pp. 169-174 More about this Journal
Abstract
Despite many efforts to improving canine in vitro maturation(IVM), the efficiency is still low compared to that of other mammalian species. The present study investigated the effects of gonadotropin, epidermal growth factor and cysteine supplementation on in vitro maturation of canine oocytes. Cumulus-oocyte complexes (COCs) were matured in basic medium (TCM-199 containing 10% FBS, 0.11mg/ml sodium pyruvate supplemented with or without $10{\mu}g/ml$ FSH, 10ng/ml EGF and 0.57mM cysteine) for 72 hr at $38.5^{\circ}C$ in humidified atmosphere of 5% $CO_2$ in air. After culture, oocytes were stained with Hoechst 33342 $(10{\mu}g/ml)$ for 30 min at $4^{\circ}C$, and assessed their nuclear status (GV: germinal vesicle, GVBD: germinal vesicle break down, MI: metaphasse I, MII: metaphase II, UK: unknown stage). No differences were observed in GV, MI and MII rate except GVBD rate between with and without gonadotropins addition respectively (6.7%, vs. 17.2%). Supplementation of 10ng/ml of EGF in hormones added IVM medium resulted in significantly (p<0.05) higher MII rate (4.54% vs.7.06%). Although there are no significantly difference, total of MI and MII rates were increased by adding cysteine. In conclusion, the present study indicates that supplementation of $10{\mu}g/ml$ LH and FSH, 10ng/ml EGF and 0.57mM cysteine in canine IVM medium show a positive influence on the progression of maturation to MII at 72 hr.
Keywords
Canine oocyte; In vitro maturation; LH; FSH; EGF; Cysteine;
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