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Analysis of Semen Parameters, Sperm Activity, and Fertility of Somatic Cell Cloned Hanwoo Bulls  

Bae, Seong-Hoon (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Hwang, Seong-Soo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Yang, Byong-Chul (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Go, Yeoung-Kyu (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Kim, Dong-Hoon (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Im, Gi-Sun (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Choi, Hwa-Sik (Department of Clinical Laboratory Science, Shin Heong College)
Jin, Dong-Il (Division of Animal Science & Resources, Chungnam National University)
Yang, Boh-Suk (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Seong, Hwan-Hoo (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Publication Information
Reproductive and Developmental Biology / v.31, no.3, 2007 , pp. 139-143 More about this Journal
Abstract
This study was performed to investigate the reproductive characteristics of the cloned Hanwoo bulls produced by SCNT. The semen ejaculated from the cloned bulls (C-38 and C-39) and normal Hanwoo bull was properly measured the volume, the number of sperm, and the viability of frozen-thawed sperm. The sperm activity was analyzed using computer assisted sperm analysis (CASA). To analyze fertilizing ability of the cloned bulls, in vitro fertilization and artificial insemination were performed using the frozen-thawed semen. There were no differences in semen volume, sperm concentration, and the viability of frozen-thawed sperm between cloned bulls and normal bull. The difference was statistically significant in total motility, curvilinear velocity (VCL), straight-line velocity (VSL), and average-path velocity (VAP) of both cloned bulls compared to those of normal Hanwoo bull, respectively (p<0.05). The cleavage and blastocyst development rate were not different between the groups. five cloned cows were artificially inseminated using the frozen-thawed semen of C-38, two of them became pregnant. Two second generation calves (one male and one female) were produced. Based on these results, the cloned Hanwoo bulls showed normal reproductive abilities of semen parameters and sperm activity to their comparators and produced cloned calves, although there are some individual differences on the parameters.
Keywords
Cloned Hanwoo Bull; Sperm motility; CASA; Fertility;
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