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Effects of BSA, PVA, Gonadotropins and Follicle Shell on In Vitro Maturation and In Vitro Fertilization of Porcine Oocytes  

Cong, Pei-Qing (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Song, Eun-Sook (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Kim, Eui-Sook (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Li, Zhao-Hua (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Zhang, Yong-Hua (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Yi, Young-Joo (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Park, Chang-Sik (Research Center for Transgenic Cloned Pigs, Chungnam National University)
Publication Information
Reproductive and Developmental Biology / v.31, no.2, 2007 , pp. 61-69 More about this Journal
Abstract
This study was designed to evaluate effects of BSA, PVA, gonadotropins and follicle shell during IVM of porcine oocytes and subsequent development to the blastocyst stage after IVF. Cumulus oocyte complexes (COCs) were cultured in TCM-199 media containing 4 mg/ml BSA and 1 mg/ml PVA during IVM for 44 hr. To compare the effect of gonadotropins on oocyte maturation, COCs were cultured with FSH+LH, FSH, LH and FSH-LH-free media during IVM. respectively. Also, different number of follicle shells (0, 2, 4 and 6) was used to examine whether the presence of follicle shell in culture medium affects oocyte maturation. The percentages of fertilization and blastocyst formation, respectively, were higher in the medium containing the PVA (49.0 and 17.9%) than those containing the BSA (40.0 and 12.2%). Significantly higher rates of Mil oocytes were in the presence of FSH+LH and FSH (88.6 and 85.1 %) compared to other treatments (64.0 and 53.4% at LH and FSH-LH-free media). Co-culture with inverted follicle shells in 2 ml maturation medium enhanced the developmental competence of porcine oocytes. In conclusion, PVA could be used as a macromolecules instead of BSA, and FSH and follicle shell played important roles in maturation of porcine oocytes.
Keywords
In vitro maturation; Gonadotropins; Follicle shell; In vitro fertilization; Porcine oocytes;
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1 Abeydeera LR, Wang WH, Cantley TC, Rieke A, Day BN (1998a): Coculture with follicular shell pieces can enhance the developmental competence of pig oocytes after in vitro fertilization: relevance to intracellular glutathione. Biol Reprod 58:213-218   DOI   ScienceOn
2 Ding J, Foxcroft GR (1994): Epidermal growth factor enhances oocyte maturation in pigs. Mol Reprod Dev 39:30-40   DOI   ScienceOn
3 Funahashi H, Kim NH, Stumpf TT, Cantley TC, Day BN (1996): Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol Reprod 54:1412-1419   DOI   ScienceOn
4 Mattioli M, Bacci ML, Galeati G, Seren E (1991): Effects of LH and FSH on the maturation of pig oocytes in vitro. Theriogenology 36:95-105   DOI   ScienceOn
5 Yamashita Y, Shimada M, Okazaki T, Maeda T, Terada T (2003): Production of progesterone from de novo-synthesized cholesterol in cumulus cells and its physiological role during meiotic resumption of porcine oocytes. Biol Reprod 68:1193-1198   DOI
6 Yi YJ, Kim MY, Lee SH, Min TS, Jin DI, Park CS (2003): Effect of cysteamine on in vitro maturation, fertilization and culture of porcine oocytes. Korean J Anim Reprod 27(4):275-280
7 Yoshida M, Ishizaki Y, Kawagishi H, bamba K, Kojima Y (1992b): Effects of pig follicular fluid on maturation and after fertilization of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J Reprod Fertil 95:481-488   DOI   ScienceOn
8 Yoshida M (1993): Role of glutathione in the maturation and fertilization in pig oocytes in vitro. Mol Reprod Dev 35:76-81   DOI   ScienceOn
9 Abeydeera LR, Day BN (1997): Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol Reprod 57: 729-734   DOI   ScienceOn
10 Coy P, Romar R, Ruiz S, Canovas S, Gadea J, Garcia VF, Matas C (2005): Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine. Reproduction 129:747-755   DOI
11 Kano K, Miyano T, Kato S (1998): Effects of glycosaminoglycans on the development of in vitro-matured and - fertilized porcine oocytes to the blastocyst stage in vitro. Biol Reprod 58:1226-1232   DOI   ScienceOn
12 Park KW, Lai L, Cheong HT, Cabot R, Sun QY, Wu GM, Rucker EB, Durtschi D, Bonk A, Samuel M, Rieke A, Day BN, Murphy CN, Carter DB, Prather RS (2002): Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts. Biol Reprod 66:1001-1005   DOI   ScienceOn
13 Funahashi H, Cantley TC, Stumpf TT, Terlouw SL, Day BN (1994a): In vitro development of in vitromatured porcine oocytes following chemical activation or in vitro fertilization. Biol Reprod 50:1072-1077   DOI   ScienceOn
14 Funahashi H, Cantley TC, Day BN (1997): Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization. Biol Reprod 57:49-53   DOI   ScienceOn
15 Grupen CG, Nagashima H, Nottle MB (1995): Cysteamine enhances in vitro development of porcine oocytes matured and fertilized in vitro. Biol Reprod 53:173-178   DOI   ScienceOn
16 Liu L, Dai Y, Moor RM (1997): Role of secreted proteins and gonadotropins in promoting full maturation of porcine oocytes in vitro. Mol Reprod Dev 47:191-199   DOI   ScienceOn
17 Nasr-Esfahani MH, Johnson MH (1992): Quantitative analysis of cellular glutathione in early preimplantation mouse embryos developing in vivo and in vitro. Hum Reprod 7:1281-1290   DOI
18 Yoshida M, Ishigaki K, Pursel VG (1992a): Effects of maturation media on male pronucleus formation in pig oocytes matured in vitro. Mol Reprod Dev 31:68-71   DOI   ScienceOn
19 Hoshino Y, Uchida M, Shimatsu Y, Miyake M, Nagao Y, Minami N, Yamada M, Mai H (2005): evelopmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig. Cloning Stem Cells 7:17-26   DOI   ScienceOn
20 Abeydeera LR, Wang WH, Prather RS, Day BN (1998b): Maturation in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro. Biol Reprod 58:1316-1320   DOI   ScienceOn
21 Miyano T, Hiro-oka RE, Kano K, Miyake M, Kusunoki H, Kato S (1994): Effects of hyaluronic acid on the development of 1- and 2-cell porcine embryos to the blastocyst stage in vitro. Theriogenology 41:1299-1305   DOI   ScienceOn
22 Tatemoto H, Muto N, Sunagawa I, Shinjo A, Nakada T (2004): Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: Role of superoxide dismutase activity in porcine follicular fluid. Biol Reprod 71:1150-1157   DOI   ScienceOn
23 Abeydeera LR (2002): In vitro production of embryos in swine. Theriogenology 57:257-273   DOI   ScienceOn
24 Mattioli M, Bacci ML, Galeati G, Seren E (1989): Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology 31:1201-1207   DOI   ScienceOn
25 Slott VL, Hales BE (1987): Effect of glutathione depletion by buthionine sulfoximineon rat embryonic development in vitro. Biochem Pharmacol 36: 683-688   DOI   ScienceOn
26 Naito K, Fukuda Y, Toyoda Y (1988): Effect of procine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete Res 21: 289-295   DOI
27 Bavister BD (1981): Analysis of culture media for in vitro fertilization and criteria of success. In: Mastroianni L, Biggera JD (eds.). Fertilization and Embryonic Development In Vitro. Plenum Press, New York, USA. pp 41-60
28 Iwasaki T, Kimura E, Totsukawa K (1999): Studies on a chemically defined medium for in vitro culture of in vitro matured and fertilized porcine oocytes. Theriogenology 51:709-720   DOI   ScienceOn
29 Wang WH, Nika K, Okuda K (1991): In vitro penetration of pig oocyts matured in culture by frozen-thawed ejaculated spermtozoa. J Reprod Fertil 93:491-496   DOI   ScienceOn
30 Wang WH, Niwa K (1995): Synergetic effects of epidermal growth factor and gonadotropins on the maturation of pig oocytes in a serum-free medium. Zygote 3:345-350   DOI
31 Park CS, Kim MY, Yi YJ, Chang YJ, Lee SH, Lee JJ, Kim MC, Jin DI (2004): Liquid boar sperm quality during storage and in vitro fertilization and culture of pig oocytes. Asian-Aust J Anim Sci 17:1369-1373   DOI
32 Shimada M, Terada T (2002): FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes. Mol Hum Reprod 8:612-618   DOI   ScienceOn
33 Brad AM, Bormann CL, Swain JE, Durkin RE, Johnson AE, Clifford AL, Krisher RL (2003): Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes. Mol Reprod Dev 64:492-498   DOI   ScienceOn
34 Ding J, Foxcroft GR (1992): Follicular heterogeneity and oocyte maturation in vitro in pigs. Biol Reprod 47:648-655   DOI   ScienceOn
35 Hagen DR, Prather RS, Sims MM, First NL (1991): Development of one-cell porcine embryos to the blastocyst stage in simple media. J Anim Sci 69: 1147-1150   DOI
36 Funahashi H, Stumpf TT, Terlouw SL, Cantley T, Rieke A, Day BN (1994b): Developmental ability of pig oocytes matured and fertilized in vitro. Theriogenology 41:1425-1433   DOI   ScienceOn
37 De Matos DG, Furnus CC, Moses DF, Baldassarre H (1995): Effect of cysteamine on glutathione level and developmental capacity of bovine oocyte matured in vitro. Mol Reprod Dev 43:432-436
38 Grupen CG, Nagashima H, Nottle MB (1997): Role of epidermal growth factor and insulin-like growth factor-I on porcine oocyte maturation and embryonic development in vitro. Reprod Fertil Dev 9:571-575   DOI   ScienceOn
39 Mattioli M, Galeati G, Seren E (1988): Effect of follicle somatic cells during pig oocyte maturation on egg penetrability and male pronuclear formation. Gamete Res 20:177-183   DOI