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Identification of Differentially Expressed Genes between Neonatal and Peripubertal Rat Thymi Using $GeneFishing^{TM}$ Polymerase Chain Reaction  

Kang, Da-Won (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and institute of Health Sciences, Gyeongsang National University)
Kim, Gyu-Tae (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and institute of Health Sciences, Gyeongsang National University)
Han, Jae-Hee (Medical Research Center for Neural Dysfunction and Department of Physiology, College of Medicine and institute of Health Sciences, Gyeongsang National University)
Publication Information
Reproductive and Developmental Biology / v.31, no.1, 2007 , pp. 55-60 More about this Journal
Abstract
Aging causes thymus involution, and genes in thymus play an important role in the development of the immune system. In this study, we compared genes expressed in thymus of neonatal and peripubertal rats using annealing control primers (ACPs)-based GeneFishing polymerase chain reaction (PCR) and semiquantitative reverse transcription (RT)-PCR. We identified 10 differentially expressed genes (DEGs) with 20 ACPs. Of 10 DEGs, bystin-like, collagen type V alpha 1 (COL5A1), and T-cell receptor beta-chain segment 2 (TCRB2) that are related to immune-function were detected in rat thymus. Bystin-like and TCRB2 were up-regulated, while COL5A1 was down-regulated in peripubertal thymus. Semiquantitative RT-PCR confirmed postnatal changes in expression of bystin-like, COL5A1, and TCRB2. These results suggest that bystin-like, COL5A1, and TCRB2 could regulate immune function controlled in thymus as age increases.
Keywords
Aging; Gene expression; Polymerase chain reaction; Rats; Thymus gland;
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