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Effects of Energy Substrates in Culture Media on Developmental Capacity of Mouse Embryos  

Park, Kee-Sang (Department of OB/GYN, Kyungpook National University Hospital)
Lee, Hyun-Jung (Department of OB/GYN, Daegu Fatima Hospital)
Park, Sung-Baek (Department of Animal Science, Daegu University)
Kim, Ji-Chul (Department of Animal Science, Daegu University)
Lee, Taek-Hoo (Department of OB/GYN, Kyungpook National University Hospital)
Chun, Sang-Sik (Department of OB/GYN, Kyungpook National University Hospital)
Publication Information
Reproductive and Developmental Biology / v.31, no.1, 2007 , pp. 35-41 More about this Journal
Abstract
This study was conducted to examine the effects of energy substrates in different conoentration of carbohydrates in the human oviduct and uterus on the in vitro development of mouse 2-cell embryos. Two-cell embryos were collected from ICR female mice at $46{\sim}50\;hr$ after 5 IU hCG injection and cultured in three different media [control: 0 mM, Guoup A: glucose (G) 0.5 mM + pyruvate (P) 0.32 mM + lactate (L) 10.5 mM, Group B: G 3.15 mM + P 0.1 mM + L 5.83 mM] for 72 hr. Rates of morula formation of group A (72.3%) and B (56.6%) were significantly higher higher (p<0.05) than that of control (34.9%) at 24 hr. However, blastocyst rate was significantly higher (p<0.05) in control (51.8%) than group A (39.8%) and B (28.9%) at hr. At 72 hr, no differences were found in the number of zona-intact, zona-escape and total blastocysts among groups. Mean and ICM cell numbers were significantly higher (p<0.05) in group A (78.0, 13.4) and B (64.4, 11.8) than control (53.1, 5.7), respectively, The percent of ICM were significantly higher (p<0.05) in group A (22.9%) and B (23.7%) than control (14.2%). No differences were round in the TE cell numbers ($34.1{\sim}45.1$). The ICM:TE ratio was significantly higher $34.1{\sim}45.1$ in control (1:6.0) than group A (1:3.4) and B (1:3.4). This study shows that energy substrates added to culture media especially, the oviductal level of carbohydrates increase the developmental capacity of 2-cell mouse embryos.
Keywords
Energy substrates; Mouse embryos; Blastocysts; Cell numbers;
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