Browse > Article

Analysis of DNA Methyltransferases (Dnmts) Expression during Early Development  

Ko, Yeoung-Gyu (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Kim, Jong-Mu (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Im, Gi-Sun (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Yang, Byoung-Chul (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Lee, Hwi-Cheul (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Seong, Hwan-Hoo (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Yang, Boh-Suk (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Publication Information
Reproductive and Developmental Biology / v.30, no.4, 2006 , pp. 255-261 More about this Journal
Abstract
There are replete numbers of reports which have apparently shown that established patterns of methylation are critical for normal mammalian development. Here, we report expression of the DNA methyltransferases (Dnmts) family during mouse early development. Transcription of Dnmt1o occurs in one-cell and morula stage embryos, whereas Dnmtls transcripts were detectable in all cells and tissues examined during the study. Dnmt3a1 transcript was detected in all cells and Dnmt3a2 transcript was particularly detected in the oocyte and 1-cell stages. Low level Dnmt3b1 transcripts were expressed ubiquitously in oocyte, 1-cell, and preimplantation embryos except $2{\sim}4cell$ stages. Dnmt3b3 transcripts were only detected in E7.5 embryo and ovary. Furthermore, Dnmt31 transcripts were detectable in all cells and tissues examined. Unlike Dnmtl, both Dnmt3a and Dnmt3b proteins existed in the nucleus of preimplantation embryos till the morula stage. These Results suggest that differences Dnmts expression level exist and genomic DNA methylation patterns may be determined partly through differential expression of Dnmts during early development.
Keywords
Methylation; Dnmt1; Dnmt3a; Dnmt3b; Dnmt31;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Aoki F, Hara KT, Schultz RM (2003): Acquisition of transcriptional competence in the 1-cell mouse embryo: requirement for recruitment of maternal mRNAs. Mol Reprod Dev 64:270-274   DOI   ScienceOn
2 Bestor TH, Verdine GL (1994): DNA methyltransferases. Curr Opin Cell Biol 6: 380-389   DOI   ScienceOn
3 Caspary T, Cleary MA, Baker CC, Guan XJ, Tilghman SM (1998): Multiple mechanisms regulate imprinting of the mouse distal chromosome 7 gene cluster. Mol Cell BioI 18:3466-3474   DOI
4 Chen T, Ueda Y, Xie S, Li E (2002): A novel Dnmt 3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem 277:38746-38754   DOI   ScienceOn
5 Chung YG, Ratnam S, Chaillet JR, Latham KE (2003): Abnormal regulation of DNA methyltransferase expression in cloned mouse embryos. Biol Reprod 69: 146-153   DOI   ScienceOn
6 DeBaun MR, Niemitz EL, Feinberg AP (2003): Association of in vitro fertilization with Beckwith-Wiedemann syndrome and epigenetic alterations of LIT1 and H19. Am J Hum Genet 72:156-160   DOI   ScienceOn
7 Okano M, Bell DW, Haber DA, Li E (1999): DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247-257   DOI   ScienceOn
8 Ratnam S, Mertineit C, Ding F, Howell CY, Clarke HJ, Bestor TH, Chaillet JR, Trasler JM (2002): Dynamics of Dnmtl methyltransferase expression and intracellular localization during oogenesis and preimplantation development. Dev Biol 245:304-314   DOI   ScienceOn
9 Chen T, Ueda Y, Dodge JE, Wang Z, Li E (2002): Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell BioI 23:5594-5605
10 Hata K, Okano M, Lei H, Li E (2002): Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice. Development 129:1983-1993
11 Tate PH, Bird AP (1993): Effects of DNA methylation on DNA-binding proteins and gene expression. Curr Opin Genet Dev 3:226-231   DOI   ScienceOn
12 Santos F, Hendrich B, Reik W, Dean W (2002): Dynamic reprogramming of DNA methylation in the early mouse embryo. Dev Biol 241:172-182   DOI   ScienceOn
13 Carlson LL, Page AW, Bestor TH (1992): Properties and localization of DNA methyltransferase in preimplantation mouse embryos: implications for genomic imprinting. Genes Dev 6:2536-2541   DOI   ScienceOn
14 Ko YG, Nishino K, Hattori N, Arai Y, Tanaka S, Shiota K (2005): Stage-by-stage change in DNA methylation status of Dnmtl locus during mouse early development. J BioI Chem 280:9627-9634   DOI
15 Mertineit C, Yoder JA, Taketo T, Laird DW, Trasler JM, Bestor TH (1998): Sex-specific exons control DNA methyltransferase in mammalian germ cells. Development 125:889-897
16 Aapola V, Liiv I, Peterson P (2002): Imprinting regulator DNMI3L is a transcriptional repressor associated with histone deacetylase activity. Nucleic Acids Res 30:3602-3608   DOI   ScienceOn
17 Bourc'his D, Xu GL, Lin CS, Bollman B, Bestor TH (2001): Dnmt3L and the establishment of maternal ge- nomic imprints. Science 294:2536-2539   DOI
18 Weisenberger DJ, Velicescu M, Preciado-Lopez MA, Gonzales FA, Tsai YC, Liang G, Jones PA (2002): Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells. Gene 298:91-99   DOI   ScienceOn
19 Yin B, Chen Y, Zhu N, Wu G, Sheri Y (1999): Cloning of full-length Dnmt3b cDNA and its alternative splicing isoforms in mouse embryo]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21:431-438
20 Ng HH, Zhang Y, Hendrich B, Johnson CA, Turner BM, Erdjument-Bromage H, Tempst P, Reinberg D, Bird A (1999): MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. Nat Genet 23:58-61
21 Chedin F, Lieber MR, Hsieh CL (2002): The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a. Proc Natl Acad Sci USA 99:16916-16921
22 Monk M, Boubelik M, Lehnert S (1987): Temporal and regional changes in DNA methylation in the embryonic, extraembryonic and germ cell lineages during mouse embryo development. Development 99:371-382
23 Pradhan 5, Bacolla A, Wells RD, Roberts RJ (1999): Recombinant human DNA (cytosine-5) methyl transferase. I. Expression, purification, and comparison of de novo and maintenance methylation. J Biol Chem 274:33002-33010   DOI
24 Okano M, Xie S, Li E (1998b): Dnmt2 is not re-quired for de novo and maintenance methylation of viral DNA in embryonic stem cells. Nucleic Acids Res 26:2536-2540   DOI   ScienceOn
25 Bird AP, Wolffe AP (1999): Methylation-induced repression-belts, braces, and chromatin. Cell 99:451-454   DOI   ScienceOn
26 Cox GF, Burger J, Lip V, Mau UA, Sperling K, Wu BL, Horsthemke B (2002): Intracytoplasmic sperm injection may increase the risk of imprinting defects. Am J Hum Genet 71:162-164   DOI   ScienceOn
27 Cardoso MC, Leonhardt H (1999): DNA methyltransferase is actively retained in the cytoplasm during early development. J Cell Biol 147:25-32   DOI   ScienceOn
28 Gruenbaum Y, Stein R, Cedar H, Razin A (1981): Methylation of CpG sequences in eukaryotic DNA. FEBS Lett 124:67-71   DOI   ScienceOn
29 Kass SU, Pruss D, Wolffe AP (1997): How does DNA methylation repress transcription? Trends Genet 13: 444-449   DOI   ScienceOn
30 Okano M, Xie S, Li E (1998a): Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nat Genet 19:219-220   DOI   ScienceOn
31 Howell CY, Bestor TH, Ding F, Latham KE, Mertineit C, Trasler JM, Chaillet JR (2001): Genomic imprinting. disrupted by a maternal effect mutation in the Dnmtl gene. Cell 104:829-838   DOI   ScienceOn
32 Shiota K, Kogo Y, Ohgane J, Imamura T, Urano A, Nishino K, Tanaka S, Hattori N (2002): Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice. Genes Cells 7:961-969   DOI   ScienceOn
33 Li E, Beard C, Jaenisch R (1993): Role for DNA methylation in genomic imprinting. Nature 366:362-365   DOI   ScienceOn
34 Yoder JA, Bestor TH (1998): A candidate mammalian DNA methyltransferase related to pmtlp of fission yeast. Hum Mol Genet 7:279-284   DOI   ScienceOn
35 Kimura H, Takeda T, Tanaka S, Ogawa T, Shiota K (1998): Expression of rat DNA (cytosine-5) methyltransferase (DNA Mfase) in rodent trophoblast giant cells: Molecular cloning and characterization of rat DNA Mfase. Biochem Biophys Res Commun 253: 495-501   DOI   ScienceOn
36 Aoki A, Suetake I, Miyagawa J, Fujio T, Chijiwa T, Sasaki H, Tajima S (2001): Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases. Nucleic Acids Res 29:3506-3512   DOI   ScienceOn
37 Li E, Bestor TH, Jaenisch R (1992): Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69:915-926   DOI   ScienceOn
38 Bestor T, Laudano A, Mattaliano R, Ingram V (1988): Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases. J Mol BioI 203:971-983   DOI
39 Dean W, Santos F, Stojkovic M, Zakhartchenko V, Walter J, Wolf E, Reik W (2001): Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos. Proc Natl Acad Sci USA 98:13734-13738