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Perinatal Phthalate/Adipate Esters Exposure in Rats: Effects on Maternal Body Weight Changes and Developmental Landmarks in Offspring Rats  

Lee, Hwi-Cheul (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Yang, Byoung-Chul (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Ko, Yeoung-Gyu (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Kim, Dong-Hoon (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Park, Jin-Ki (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Yang, Boh-Suk (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Seong, Hwan-Hoo (Animal Biotechnology Division, National Livestock Research Institute, RDA)
Yamanouchi, Keitaro (Department of Veterinary Physiology, Veterinary Medical Science, Tokyo University)
Nishihara, Masugi (Department of Veterinary Physiology, Veterinary Medical Science, Tokyo University)
Publication Information
Reproductive and Developmental Biology / v.30, no.4, 2006 , pp. 235-245 More about this Journal
Abstract
Di-n-butyl phthalate (DBP), diisononyl phthalate (DINP) and di-(2-ethylhexyl) adipate (DEHA) are ubiquitously distributed chemicals that are widely used as plasticizers and also found at low levels in foods. The aims of this study were to determine whether perinatal exposure to DBP, DINP and DEHA could alter normal patterns of neonatal development. Dams were provided with pulverized soy-free diet containing 20, 200, 2,000 and 10,000 ppm of DBP, 40, 400, 4.000 and 20,000 ppm of DINP, or 480, 2,400 and 12,000 ppm of DEHA from gestational day 15 to postnatal day 21. Exposure to the high doses of DBP, DINP and DEHA during gestational period significantly decreased food consumption and body weight gain of dams. These chemicals reduced neonatal body weight as well as that of the after maturation. Also, exposure to DINP of all the doses used and the higher doses (2,400 and 12,000 ppm) of DEHA decreased AGD at PND 1 in male neonates, though that to DBP did not affect AGD in males. In female neonates, an increase in AGD was observed in DBP- and DINP-exposed animals at the highest doses. Moreover, these chemicals affected survival rate of pups at PND 5, and delayed onset of eye opening in all chemica1-exposed groups at PND 17. These results suggest that perinatal exposure to these chemicals may affect the normal development and/or growth of offspring.
Keywords
Di-n-butyl phthalate (DBP); Diisononyl phthalate (DINP); Di-(2-ethylhexyl) adipate (DEHA); Developmental Landmarks; Perinatal exposure;
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