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Toxicologic Effects of BPA and DEHP on Semen Characteristics in Pig  

Park, Dong-Heon (College of Animal Life Science, Kangwon National University)
Jang, Hyun-Yong (College of Animal Life Science, Kangwon National University)
Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
Kim, Jong-Taek (School of Veterinary Medicine, Kangwon National University)
Park, Choon-Keun (College of Animal Life Science, Kangwon National University)
Kim, Choung-Ik (College of Animal Life Science, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.30, no.3, 2006 , pp. 219-224 More about this Journal
Abstract
The objective of this study was to investigate the toxic effects of Bisphenol A(BPA) and di-2 ethyhexyl phthalate (DEHP) as endocrine disrupters on sperm motility, viability, membrane integrity and abnormality during in vitro incubation of boar semen. Semen were randomly divide into 24 groups and healed with different concentrations of BPA md DEHP($1{\sim}100{\mu}M$) for 3, 6 and 9 hrs, respectively. The percentages of sperm motility and viability decreased by treatment time with both BPA and DEHP, and obiously differ from the controls. The percentages of sperm motility and viability significantly decreased by incubation with both $100{\mu}M$ of BPA and DEHP compared to control and other treatment groups(p<0.05). Sperm membrane integrity was significantly reduced by incubation with 10 and $100{\mu}M$ of BPA and DEHP, respectively(p<0.05), but sperm abnormality were not significantly affect both BPA and DEHP. These results indicate that high concentration of BPA and DEHP($>10{\mu}M$ can affect noxiously the sperm characteristics.
Keywords
BPA; DEHP; Semen characteristics; Endocrine disrupter; Pig;
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